Role of ApoB-516C/T promoter gene polymorphism in the risk of Hepatitis C virus infection in Egyptian patients and in gender susceptibility

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Khalifa R.H.; Labib D.A.; Kamel M.A.; Shahin R.M.H.; Bahgat D.M.R.; Riad N.M.; El Khateeb E.; El-deeb A.M.; Hassan M.

Journal: Journal of Medical Virology

Publisher: John Wiley and Sons Inc.

Publication Date: 12 May 2017

Volume / Issue: Volume 89 / Issue 9

Pages: 1584–1589

ISSN: 1466615

DOI: 10.1002/jmv.24815

Scopus: View on Scopus

PubMed: 28370191

Document Type: Article

Authors and Affiliations

Khalifa R.H., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Labib D.A., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Kamel M.A., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Shahin R.M.H., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Bahgat D.M.R., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Riad N.M., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; El Khateeb E., Department of Clinical Pathology, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; El-deeb A.M., Department of Tropical Medicine, Kasr Al-Ainy, School of Medicine, Cairo University, Cairo, Egypt; Hassan M., Department of Immunology, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

At least 1 in 10 of the Egyptian population aged 15-59 is burdened with hepatitis C virus (HCV) infection, stamping Egypt the highest country harboring HCV worldwide. Considerable evidence supported the involvement of host genetic factors in the pathogenesis of HCV and the possibility of implementation in target therapies. ApoB gene polymorphisms are postulated to affect the susceptibility of HCV infection. Hence, we aimed to evaluate the relationship between ApoB-516C/T promoter gene polymorphism and HCV infection in a cohort of Egyptian patients and to explore whether higher levels of low-density lipoprotein (LDL) might compete with lipoviral particles (LVP) in the binding to LDL receptor (LDLR), thus escaping infection. Ninety-seven HCV patients and 96 matched controls were enrolled in this study. We genotyped ApoB-516C/T using PCR-RFLP method. ApoB concentrations were measured by immunoturbidimetric assay. The genotype and the allele frequencies of ApoB-516C/T promoter gene polymorphism in cases were statistically insignificant compared with healthy individuals (P = 0.109, 0.125, respectively). Sex stratification showed significantly lower counts of C/T genotype in female patients compared with female controls (P = 0.011, OR = 0.132, 95% CI = 0.026-0.657). Significantly higher levels of LDL and ApoB were detected in the control group (P < 0.001). This study shows that the ApoB-516C/T promoter gene polymorphism has no impact on the risk of HCV infection. However, the C/T genotype may be a protective factor for our female cohort. Further studies with larger samples are needed to verify this genetic gender diversity. Additionally, high levels of LDL and ApoB might prevent HCV infection. © 2017 Wiley Periodicals, Inc.

Keywords

ApoB promoter gene polymorphism; Egypt; HCV; LDLR; LVP; Adolescent; Adult; Apolipoprotein B-100; Cohort Studies; Female; Genetic Predisposition to Disease; Genotyping Techniques; Hepatitis C; Humans; Male; Middle Aged; Nephelometry and Turbidimetry; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Risk Assessment; Sex Factors; Young Adult; apolipoprotein B; low density lipoprotein; low density lipoprotein receptor; APOB protein, human; apolipoprotein B100; ApoB gene; Article; cohort analysis; controlled study; Egyptian; gender; gene frequency; genetic polymorphism; genetic risk; genetic susceptibility; genotype; human; infection risk; major clinical study; promoter region; virus particle; blood; genetic predisposition; genetics; genotyping technique; photometry; restriction fragment length polymorphism; sex factor; single nucleotide polymorphism

Citation Information

Scopus Citations: 8

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