Expression of ERG protein and TMRPSS2-ERG fusion in prostatic carcinoma in Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdel-Hady A.; El-Hindawi A.; Hammam O.; Khalil H.; Diab S.; Abd El-Aziz S.; Badawy M.; Ismail A.; Helmy N.; Kamel N.; Anis S.; El Kholy A.; Al Osili K.; Abdel-Hady A.; Nour H.; Akl M.

Journal: Open Access Macedonian Journal of Medical Sciences

Publication Date: 20 March 2017

Volume / Issue: Volume 5 / Issue 2

Pages: 147–154

ISSN: 18579655

DOI: 10.3889/oamjms.2017.037

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abdel-Hady A., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; El-Hindawi A., Faculty of Medicine Cairo University, Cairo, Egypt; Hammam O., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Khalil H., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Diab S., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Abd El-Aziz S., Faculty of Medicine Cairo University, Cairo, Egypt; Badawy M., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Ismail A., Faculty of Medicine Cairo University, Cairo, Egypt; Helmy N., National Research Center El Doki, Giza, Egypt; Kamel N., National Research Center El Doki, Giza, Egypt; Anis S., Faculty of Medicine Cairo University, Cairo, Egypt; El Kholy A., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Al Osili K., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Abdel-Hady A., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Nour H., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; Akl M., Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt

Abstract

AIM: Prostate cancer (PCa) is the second most common cancers in men worldwide. Its incidence can be influenced by several risk factors including genetic susceptibility. Therefore the search for the expression of a certain gene (ERG) and its rearrangement could give us clues for proper identification of PCa. And the study of ERG expression and its comparison to FISH in Egyptian patients can show whether ERG immunophenotype could be used instead of FISH, as it is cheaper. MATERIALS AND METHODS: This study was performed on 85 cases of PCa, showing 30 cases with HGPIN and 30 cases of prostatic hyperplasia. All were immunohistochemistry stained using ERG monoclonal rabbit antihuman antibody was used (clone: EP111). FISH analysis was performed in 38 biopsies of PCa cases to detect TMRPSS2-ERG rearrangement using the FISH ZytoLight TriCheck Probe (SPEC TMRPSS2-ERG). RESULTS: ERG expression was found in 26% of PCa cases and 20% of HGPIN cases. FISH analysis showed fusion of 21 cases of PCa (out of 22 cases showing ERG immunoexpression). CONCLUSION: Our findings emphasise that only malignant and pre-malignant cells and not benign cells from the prostate stain positive. ERG expression may offer a simpler, accurate and less costly alternative for evaluation of ERG fusion status in PCa. © 2017 Ahmed Abdel-Hady, Ali El-Hindawi, Olfat Hammam, Heba Khalil, Sara Diab, Soulafa Abd El- Aziz, Mohamed Badawy, Ahmed Ismail, Noha Helmy, Nora Kamel, Shady Anis, Amr El Kholy, Khalid Al Osili, Afaf Abdel-Hady, Hani Nour, Maha Akl.

Keywords

ERG Immunoexpression; Fluorescence in situ hybridization (FISH); High grade prostatic intraepithelial neoplasia; Prostatic carcinoma; TMRPSS2-ERG; transcription factor ERG; adult; aged; Article; chromosome translocation; controlled study; Egyptian; female; fluorescence in situ hybridization; gene deletion; gene expression; gene overexpression; genetic susceptibility; hemangioma; histopathology; human; human tissue; immunohistochemistry; major clinical study; male; prostate cancer; prostate hypertrophy; protein expression

Citation Information

Scopus Citations: 6

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