Diagnostic biomarkers in murine Cryptosporidiosis: dose- and age-related infection

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Yousof H.-A.S.; Khater M.M.; El-Sayed S.H.; El-Badry A.A.

Journal: Journal of Parasitic Diseases

Publisher: Springer India

Publication Date: 18 February 2017

Volume / Issue: Volume 41 / Issue 3

Pages: 831–836

ISSN: 9717196

DOI: 10.1007/s12639-017-0898-2

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Bronze Open Access; Green Open Access

Authors and Affiliations

Yousof H.-A.S., Medical Parasitology Department, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, 1156, Elmanial, Egypt; Khater M.M., Medical Parasitology Department, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, 1156, Elmanial, Egypt; El-Sayed S.H., Department of Medical Parasitology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; El-Badry A.A., Medical Parasitology Department, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, 1156, Elmanial, Egypt

Abstract

Increasing prevalence of Cryptosporidium raises the importance to explore different aspects of its infection. In the absence of reproducible in vitro culturing, animal model is the only experimental method to study Cryptosporidium. Our study evaluated Cryptosporidium infection using coproscopy, copro-antigen and copro-DNA for early detection of murine cryptosporidiosis. Hundred and forty albino mice (neonates and adult) were divided into two groups, control group received sterile PBS solution, and infected groups were inoculated with molecularly characterized Cryptosporidium parvum oocysts and further subdivided into three subgroups for infectious dose response detection. Mice fecal samples were collected every 4 h on the first day and then daily and examined for fecal oocysts, copro-antigen and copro-DNA. Four mice from each subgroup were killed at 12, 24 and 48 h post-infection (P-I), and their intestines were examined for cryptosporidial mucosal DNA. Cryptosporidium copro-antigen and copro-DNA were detected 4 and 8 h P-I in infected neonatal and adult mice, respectively, and intestinal mucosal DNA was detected after 12 h in both. Microscopy was able to detect oocysts 48 h P-I. Inoculated C. parvum oocysts were recovered in feces of infected mice without genotypic changes. Neonate mice showed higher susceptibility for cryptosporidial infection than adults without statistical differences for the given infectious doses. Both copro-immunoassay and copro-nPCR assays can early detect Cryptosporidium infection; however, nPCR was able to identify Cryptosporidium species, making nPCR a reliable biomarker for early detection in murine model. © 2017, Indian Society for Parasitology.

Keywords

Copro-DNA; Cryptosporidium; Diagnostic biomarker; Fecal immunoassay; Murine; Nested PCR; biological marker; adult; animal experiment; Article; controlled study; cryptosporidiosis; Cryptosporidium parvum; DNA extraction; DNA isolation; immunoassay; male; mouse; newborn; nonhuman; polymerase chain reaction; priority journal

Citation Information

Scopus Citations: 4

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