Gas chromatography-mass spectrometry analysis, antimicrobial, anticancer and antioxidant activities of n-hexane and methylene chloride extracts of Senna italica

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Madkour H.M.F.; Ghareeb M.A.; Abdel-Aziz M.S.; Khalaf O.M.; Saad A.M.; El-Ziaty A.K.; Abdel-Mogib M.

Journal: Journal of Applied Pharmaceutical Science

Publisher: Open Science Publishers LLP Inc.

Publication Date: 2017

Volume / Issue: Volume 7 / Issue 6

Pages: 23–32

ISSN: 22313354

DOI: 10.7324/JAPS.2017.70604

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Madkour H.M.F., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt; Abdel-Aziz M.S., Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Center, El Behoos Street 33, Dokki-Giza, Egypt; Khalaf O.M., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; Saad A.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt; El-Ziaty A.K., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; Abdel-Mogib M., Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Mansoura, 35516, Egypt

Abstract

The current study was aimed to investigate the antimicrobial, anticancer, and antioxidant activities of the n-hexane and methylene chloride extracts from Senna italica as well as the characterization of their chemical constituents via gas chromatography-mass spectrometry analysis (GC-MS). The results revealed that the n-hexane extract showed weak antimicrobial activity with inhibition zones were ranged from 3.8 to 7mm, and the methylene chloride extract showed a potent antimicrobial activity with inhibition zones were ranged from 4.2 to 18mm compared to five standard antibiotics. Also, according to the criteria of the American National Cancer Institute (USNCI), the n-hexane showed weak anticancer activity with IC50 equal to 81.6±4.1, 73.0±3.90, 66.8±3.12, and 92.8±4.82 μg/ml, while the methylene chloride exhibited a potent anticancer activity with IC50 of 16.9±1.30, 17.4±1.36, 18.3±1.59, and 14.2±1.18μg/ml, compared to 5-fluorouracil with IC50 of 7.9±0.28, 4.8±0.21, 8.3±0.35, and 5.4±0.20, all respectively for HePG-2, Hela, PC3, and MCF-7 tumor cell lines. The activity against ABTS radical was recorded with the methylene chloride (% inhibition= 86.3%), compared to ascorbic acid with 89.2%. Moreover, GC-MS analysis revealed that the major constituents of the n-hexane were n-hexadecanoic acid (30%), (Z,Z,Z)9,12,15-octadecadienoic acid (21%), vitamin E (7.32%), and for methylene chloride were 3-methyl-4-oxopentanoic acid (16.36%), (E)-stilbene (11.86%), and 2,6-di-tert-butylphenol (10.70%). © 2017 Hassan M.F. Madkour et al.

Keywords

ABTS; Antimicrobial; anticancer; GC-MS analysis; Senna italica

Citation Information

Scopus Citations: 49

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