Differential expression of glypican-3 and insulin-like growth factor-II mRNAs and alpha-fetoprotein and Ki-67 markers in HCV related hepatocellular carcinomas in Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Saber M.A.; AbdelHafiz S.M.M.; Khorshed F.E.; Aboushousha T.S.; Hamdy H.E.M.; Seleem M.I.; Soliman A.H.

Journal: Asian Pacific Journal of Cancer Prevention

Publisher: Asian Pacific Organization for Cancer Prevention

Publication Date: 2017

Volume / Issue: Volume 18 / Issue 1

Pages: 121–127

ISSN: 15137368

DOI: 10.22034/APJCP.2017.18.1.121

Scopus: View on Scopus

PubMed: 28240019

Document Type: Article

Authors and Affiliations

Saber M.A., Biochemistry And Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; AbdelHafiz S.M.M., Biochemistry And Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Khorshed F.E., Biochemistry And Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Aboushousha T.S., Pathology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Hamdy H.E.M., Surgery Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Seleem M.I., Hepato-Pancreatic-Biliary Surgery Department, National Hepatology and Tropical Medicine Institute (NHTMRI), Cairo University, Cairo, Egypt; Soliman A.H., Clinical Pathology Department, National Cancer Institute (NCI), Cairo University, Cairo, Egypt

Abstract

Background: Increasing evidence indicates that in hepatocellular carcinomas (HCCs) abnormal gene expression, for example of glypican-3 (GPC-3) and insulin-like growth factor-II (IGF-II), are associated with the occurrence and progression of HCC. The objective of this study was to evaluate the differential expression of GPC-3 and IGF-II mRNAs in HCC tissues with a background of chronic hepatitis C virus (HCV) genotype 4 cirrhosis, in relation to Ki-67 and alpha-feto protein (AFP) tissue markers. Methods: One hundred and five patients with HCCs who had undergone hepatectomy, were included, after obtaining informed consent. Total RNA was extracted from malignant and corresponding peri-malignant liver tissues, and GPC-3 and IGF-II mRNAs in addition to beta-actin mRNA as an internal control, were evaluated in all samples by reverse transcriptase-polymerase chain reactions (RT-PCR). Routine histopathological diagnosis as well as immunohistochemical (IHC) staining using monoclonal antibodies for Ki-67 and AFP were also performed. Result: Expression of GPC-3 mRNA was positive in all HCC malignant tissue, with overexpression in 86/105 (81.9%); in respect to the grade of the tumor (1-3 grades), while in peri-malignant tissue it was over expressed only in 20/105 (19%). The IGF-II mRNA was over expressed in only 10/105 (9.5%) malignant and peri-malignant samples. AFP was expressed in 33.3% of malignant samples but absent in peri-malignant tissues. Ki-67 expression was significantly increased in malignant compared to peri-malignant tissue. Conclusion: GPC-3 and IGF II mRNAs may be good molecular markers for HCC, especially with a background of cirrhosis due to chronic HCV infection. Significant correlations were noted with the pattern of AFP and Ki-67 expression.

Keywords

Alpha-fetoprotein; Glypican-3; HCV; Hepatocellular carcinoma; Insulin-Like Growth Factor-II

Citation Information

Scopus Citations: 10

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