Fibrosis in chronic hepatitis C: Correlation between immunohistochemically-assessed virus load with steatosis and cellular iron content

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Akl M.; El Hindawi A.; Mosaad M.; Montasser A.; El Ray A.; Khalil H.; Anas A.; Atta R.; Paradis V.; Abdel Hadi A.; Hammam O.

Journal: Open Access Macedonian Journal of Medical Sciences

Publication Date: 15 November 2016

Volume / Issue: Volume 4 / Issue 4

Pages: 578–584

ISSN: 18579655

DOI: 10.3889/oamjms.2016.122

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Akl M., Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; El Hindawi A., Department of Pathology, Faculty of Medicine Cairo University, Cairo, Egypt; Mosaad M., Department of Pathology, Faculty of Medicine Cairo University, Cairo, Egypt; Montasser A., Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; El Ray A., Department of Gastroenterology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Khalil H., Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Anas A., Department of Gastroenterology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Atta R., Department of Gastroenterology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Paradis V., Deparment of Pathology, Beaujon Hospital, Clichy, France; Abdel Hadi A., Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Hammam O., Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt

Abstract

AIM: We aimed study impact of hepatocytic viral load, steatosis, and iron load on fibrosis in chronic hepatitis C and role of VEGF and VEGFR overexpression in cirrhotic cases in evolving HCC. MATERIAL AND METHODS: Total of 120 cases were included from TBRI and Beaujon Hospital as chronic hepatitis C (CHC), post-hepatitis C cirrhosis, and HCC. Cases of CHC were stained for Sirius red, Prussian blue and immunohistochemically (IHC) for HCV-NS3/NS4. HCC were stained IHC for VEGF and by FISH. RESULTS: Stage of fibrosis was significantly correlated with inflammation in CHC (P < 0.01). Noticed iron load did not correlate with fibrosis. Steatosis was associated with higher inflammation and fibrosis. The cellular viral load did not correlate with inflammation, steatosis or fibrosis. VEGF by IHC was significantly higher in cases of HCC when compared to cirrhotic group (P < 0.001). Amplification of VEGFR2 was confirmed in 40% of cases of HCC. Scoring of VEGF by IHC was the good indicator of VEGFR2 amplification by FISH (P < 0.005). CONCLUSION: Grade of inflammation is the factor affecting fibrosis in CHC. The degree of liver damage is not related to cellular viral load or iron load. Steatosis is associated with higher inflammation and fibrosis. VEGF by IHC is correlated with overexpression of VEGFR2 by FISH. © 2016 Maha Akl, Ali EL Hindawi, Maha Mosaad, Ahmed Montasser, Ahmed El Ray, Heba Khalil, Amgad Anas, Raffat Atta, Valerie Paradis, Ahmed Abdel Hadi, Olfat Hammam.

Keywords

Fibrosis; FISH; HCC; HCV; NS3/NS4; VEGF; VEGFR2; iron; vasculotropin; vasculotropin receptor 2; Article; chronic hepatitis C; controlled study; fluorescence in situ hybridization; gene amplification; gene overexpression; histochemistry; human; human tissue; immunohistochemistry; iron overload; liver cell; liver cell carcinoma; liver fibrosis; major clinical study; microphotography; steatosis; virus load

Citation Information

Scopus Citations: 1

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