Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdel-Lateef E.; Mahmoud F.; Hammam O.; El-Ahwany E.; El-Wakil E.; Kandil S.; Abu Taleb H.; El-Sayed M.; Hassenein H.

Journal: Acta Pharmaceutica

Publisher: Hrvatsko Farmaceutsko Drustvo

Publication Date: 29 June 2016

Volume / Issue: Volume 66 / Issue 3

Pages: 387–398

ISSN: 13300075

DOI: 10.1515/acph-2016-0028

Scopus: View on Scopus

PubMed: 27383887

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abdel-Lateef E., Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; Mahmoud F., Immunology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; Hammam O., Pathology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; El-Ahwany E., Immunology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; El-Wakil E., Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; Kandil S., Immunology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; Abu Taleb H., Environmental Research Laboratories, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; El-Sayed M., Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt; Hassenein H., Immunology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar Imbaba (P.O. 30), Giza, 12661, Egypt

Abstract

The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes. © by Ezzat Abdel-Lateef 2016.

Keywords

anticancer activity; Curcuma longa L.; curcumin; GC-MS analysis; Antineoplastic Agents, Phytogenic; Apoptosis; Carcinoma, Hepatocellular; Cell Proliferation; Cell Survival; Chromatography, High Pressure Liquid; Chromatography, Liquid; Curcuma; Cyclohexenes; Drug Discovery; Gas Chromatography-Mass Spectrometry; Hep G2 Cells; Humans; Inhibitory Concentration 50; Ketones; Liver Neoplasms; Monoterpenes; Plant Extracts; Rhizome; Sesquiterpenes; Volatile Organic Compounds; 2 ethenyl 1,1 dimenthyl 3 methylene cyclohexane; 2 isopropylidene 3 methylhexa 3,5 dienal; 2 methoxy 4 vinyl phenol; absinthol; alpha cedrene; alpha cubebene; alpha curcumene; alpha turmerone; antineoplastic agent; ar curcumene; benzene 2 methyl 1,4 bis(1 methylethyl); beta bisabolene; beta cedrene; beta curcumene; beta elemene; beta himachalene; beta sesquiphellandrene; beta vatirenene; caryophyllene; cis para menth 2,8 dienol; cis sabinol; Curcuma longa extract; curlone; germacrone; hemellitol; m eugenol; o cymene; para cymene; thymol; unclassified drug; unindexed drug; ar-turmerone; beta-phellandrene; curcumenol; cyclohexene derivative; ketone; plant extract; sesquiterpene; terpene; turmeric extract; volatile organic compound; antineoplastic activity; Article; cancer chemotherapy; cancer inhibition; cell viability; chemical composition; controlled study; Curcuma longa; cytotoxicity; hepatoma cell; high performance liquid chromatography; human; human cell; IC50; in vitro study; liquid chromatography; liver cell carcinoma; mass spectrometry; chemistry; comparative study; drug development; drug effects; Hep-G2 cell line; isolation and purification; mass fragmentography; pathology

Citation Information

Scopus Citations: 87

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