Isolation and characterization of the bioactive phenolic compounds from morus alba leaves growing in Egypt

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Ghareeb M.A.; Ahmed W.S.; Refahy L.A.; Abdou A.M.; Hamed M.M.; Abdel-Aziz M.S.

Journal: Pharmacologyonline

Publisher: SILAE (Italo-Latin American Society of Ethnomedicine)

Publication Date: 2016

Volume / Issue: Volume 3

Pages: 157–167

ISSN: 18278620

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Ahmed W.S., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt, Department of Chemistry, College of Science and Arts, Sajir, Shaqra University, Saudi Arabia; Refahy L.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Abdou A.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Hamed M.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Abdel-Aziz M.S., Microbial Chemistry Department, National Research Center, Dokki, Giza, Egypt

Abstract

Morus alba Linn. (Whit Mulberry) is known for its medicinal and nutritive benefits. In the current study, different extracts of Morus alba leaves were investigated for their antioxidant activities which was evaluated via phosphomolybdenum & reducing power, and dot-blot-DPPH staining assays, as well as their total phenolic contents (TPCs). Also, their antimicrobial activity was evaluated via disc diffusion technique toward four human pathogenic strains including; Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. The results revealed that TPCs were ranged from 26.53 to 387.50 (mg gallic acid equivalent GAE/g dry extract), the total antioxidant capacity values were ranged from 47.06 to 430.50 (mg AAE/g dry extract), whereas the reducing power activities (OD values) were ranged from 0.102 to 0.659, compared to ascorbic acid of OD= 0.915. Moreover, the antimicrobial results revealed that the EtOAc extract was the strongest one against three strains with inhibition zones between 16-21 mm, followed by n-BuOH with inhibition zones between 10-18 mm. Furthermore, the chromatographic isolation of the n-BuOH upon polyamide column resulted in the isolation of six compounds, their structures were determined on the basis of 1D (1H,13C-NMR), and IR spectroscopy as; sinapic acid-O-glucoside (1), 3,4,5-trimethyoxyphenol-1-O-β-D-rhamnopyranoside (2), chlorogenic acid (3), gallic acid (4), rutin (5), and quercetin. Furthermore, the free radical antioxidant activities (DPPH) SC50 values of the compounds 1-6, were found to be 7.45, 11.65, 6.55, 5.20, 9.0, and 3.45 μg/ml respectively, relative to ascorbic acid as positive standard (7.80 μg/ml), whereas that of the total antioxidant capacity values were 560.40, 410.25, 570.15, 600, 535.50, and 630.23 (mg AAE/g compound) respectively. This is the first report of the compounds 1, 2 being isolated from the leaves of Morus alba growing in Egypt. © 2016, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.

Keywords

Antimicrobial; Antioxidant; Morus alba L.; Phenolics; Sinapic acid-O-glucoside; 3,4,5 trimethyoxyphenol 1 o beta dextro rhamnopyranoside; antiinfective agent; ascorbic acid; chlorogenic acid; gallic acid; penicillin G; phenol derivative; quercetin; rutoside; sinapic acid o glucoside; unclassified drug; antimicrobial activity; antioxidant activity; Article; Aspergillus niger; Candida albicans; carbon nuclear magnetic resonance; controlled study; drug identification; drug isolation; drug screening; Egypt; infrared spectroscopy; Morus alba; nonhuman; plant leaf; proton nuclear magnetic resonance; Pseudomonas aeruginosa; Staphylococcus aureus

Citation Information

Scopus Citations: 11

For comprehensive information about the Theodor Bilharz Research Institute (TBRI), its institutional activities, scientific and research achievements, clinical and hospital services, and the diverse expertise offered through its 22 specialized research and clinical departments, as well as opportunities for professional training, specialized workshops, and scientific conferences, readers are invited to visit the Institute’s official website.

The website provides regularly updated information on the Institute’s latest news, research activities, scientific initiatives, clinical services, institutional programs, and academic and professional opportunities.

English Website: https://www.tbri.sci.eg/en/

Arabic Website: https://www.tbri.sci.eg/ar/

Prepared and Uploaded by:

Abdalla F. Abdalla

Electronic Portal Unit

Electronic Portal Unit

Popular Posts

A cost-performance index for nano-optical biosensor evaluation: Systematic evaluation of europium–salicylate luminescent platforms for GPC3-targeted early HCC diagnosis

Image
Bibliographic Information Authors: Hassan Y.M.; El-Tantawi H.; Ali I.R.; Attia M.S. Journal: Talanta Publisher: Elsevier B.V. Publication Date: January 2027 Volume / Issue: Volume 311 Article No.: 130103 ISSN: 399140 DOI: 10.1016/j.talanta.2026.130103 Scopus: View on Scopus PubMed: 42391698 Document Type: Review Authors and Affiliations Hassan Y.M., Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt; El-Tantawi H., Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt; Ali I.R., Department of Immunology and Treatment Evaluation, Theodore Bilharz Research Institute, Giza, Egypt; Attia M.S., Chemistry Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, 11623, Saudi Arabia Abstract Comparative evaluation of nano-optical biosensor platforms lacks a standardised, multi-parameter quantitative framework that integrates analytical performance...
Read more

Interpretation of liver stiffness measurement in patients with mixed liver disease etiologies

Image
Bibliographic Information Authors: El Ray A.; El Ghannam M.; Rautou P.E. Journal: Arab Journal of Gastroenterology Publisher: Elsevier Ltd Publication Date: August 2026 ISSN: 16871979 DOI: 10.1016/j.ajg.2026.05.002 Scopus: View on Scopus PubMed: 42362465 Document Type: Letter Authors and Affiliations El Ray A., Theodor Bilharz Research Institute, Hepatogastroenterology Department, Giza, Egypt, Faculty of Medicine, October 6 University, Egypt; El Ghannam M., Theodor Bilharz Research Institute, Hepatogastroenterology Department, Giza, Egypt; Rautou P.E., Université Paris-Cité, Inserm, Centre de recherche sur l’inflammation, UMR 1149, Paris, France, AP-HP, Hôpital Beaujon, Service d’Hépatologie, DMU DIGEST, Centre de Référence des Maladies Vasculaires du Foie, FILFOIE, ERN RARE-LIVER, Clichy, France Abstract Vibration-controlled transient elastography (VCTE)–based liver stiffness measurement (LSM) is widely used for non-invasive assessment of liver fibrosis and p...
Read more

Variable clinical presentations of pulmonary hydatid cysts: a four-case series from a single center in United Arab Emirates, non-endemic region

Image
Bibliographic Information Authors: Yehya M.; Abdallah M.F.H.; Ajeka S.; Al-Masalmeh F.H.; Albaghdadi M.O.J. Journal: Journal of Surgical Case Reports Publisher: Oxford University Press Publication Date: 29 July 2026 Volume / Issue: Volume 2026 / Issue 7 Article No.: rjag658 ISSN: 20428812 DOI: 10.1093/jscr/rjag658 Scopus: View on Scopus Document Type: Article Access: All Open Access; Gold Open Access; Green Open Access Authors and Affiliations Yehya M., Department of Thoracic Surgery, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates; Abdallah M.F.H., Department of Radiology, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates, Theodor Bilharz Research Institute, Mahad Al Abhas Al bahari St, Kornish Al Nile, Warraq Al Arab, Al Warak, Governorate, Giza, 3863341, Egypt; Ajeka S., Department of General Surgery, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates; Al-Masalmeh F.H., Department of ...
Read more

Holothuria arenicola Extract-Loaded Polycaprolactone Nanocapsules Attenuate Bile Duct Ligation-Induced Acute Liver Injury

Image
Bibliographic Information Authors: Mansour E.M.; Shemis M.; Fahmy S.R.; Amer M.A.; Mohamed A.S. Journal: Current Nanomedicine Publisher: Bentham Science Publishers Publication Date: September 2026 Volume / Issue: Volume 16 / Issue 5 Pages: 771–786 ISSN: 24681873 DOI: 10.2174/0124681873401484250924125945 Scopus: View on Scopus Document Type: Article Authors and Affiliations Mansour E.M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt; Shemis M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt; Fahmy S.R., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt; Amer M.A., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt; Mohamed A.S., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt Abstract Introduction: Cholestatic liver diseases are characterized by jaundice and cholestasis with various conseq...
Read more

Mytilus edulis-mediated green synthesis of selenium nanoparticles with antimicrobial and molluscicidal applications

Image
Bibliographic Information Authors: Dokmak H.-A.A.; AlSedawy M.; Ramadan M.A.; Amin B.H.; Faid A.H.; Abdelmaksoud H.F.; El-Sayed S.M. Journal: Scientific Reports Publisher: Nature Research Publication Date: 7 September 2026 Volume / Issue: Volume 16 / Issue 1 Article No.: 27918 ISSN: 20452322 DOI: 10.1038/s41598-026-65860-3 Scopus: View on Scopus PubMed: 42706305 Document Type: Article Access: All Open Access; Gold Open Access; Green Open Access Authors and Affiliations Dokmak H.-A.A., Department of Medical Malacology, Theodor Bilharz Research Institute, Giza, Egypt; AlSedawy M., Microbiology and Immunology Department, Faculty of Pharmacy (For Boys), Al-Azhar University, Cairo, Egypt; Ramadan M.A., Department of Laser Application in Metrology, Photochemistry and Agriculture, National Institute of Laser Enhanced Science (NILES) Cairo University (CU), Giza, Egypt; Amin B.H., The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, 11787,...
Read more

Corrigendum to ‘Eco-friendly approach for the removal and simultaneous detection of cyanide toxins from drinking and wastewater sources’ [Environ. Pollut. 385 (2025) 127094]

Image
Bibliographic Information Authors: El-Sewify I.M.; Shenashen M.A.; Gomaa H.; Selim M.S.; Akhtar N.; Azzam A.; Mekawy M.; Emran M.Y.; Khairy M.; Shahat A.; Hasan M.; Elmarakbi A.; El-Safty S.A. Journal: Environmental Pollution Publisher: Elsevier Ltd Publication Date: September 2026 Volume / Issue: Volume 405 Article No.: 128412 ISSN: 2697491 DOI: 10.1016/j.envpol.2026.128412 Scopus: View on Scopus PubMed: 42259729 Document Type: Erratum Authors and Affiliations El-Sewify I.M., Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), 1–2-1 Sengen, Ibaraki-ken, Tsukubashi, 305–0047, Japan, Chemistry Department, Faculty of Science, Ain Shams University, Abbasia, Cairo, 11566, Egypt; Shenashen M.A., Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), 1–2-1 Sengen, Ibaraki-ken, Tsukubashi, 305–0047, Japan; Gomaa H., Research Center for Macromolecules and Biomaterials, Na...
Read more

Multifunctional polycaprolactone-based composite fibers with icariin and glutathione for effective repair of critical-sized bone defects

Image
Bibliographic Information Authors: Yessuf A.M.; Zhang Y.; Sharma B.P.; Wang Y.; Kassa T.S.; Qadir A.; Jinqiu Y.; Mohideen M.M.; Salama A.M.; Wang W.; Zhang Q.; Liu Y. Journal: BMEMat Publisher: John Wiley and Sons Inc Publication Date: 30 July 2026 ISSN: 27517438 DOI: 10.1002/bmm2.70115 Scopus: View on Scopus Document Type: Article Access: All Open Access; Gold Open Access Authors and Affiliations Yessuf A.M., Key Laboratory of Advanced Functional Polymer Composites, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, China; Zhang Y., Henan Provincial Orthopedic Hospital, Henan, Zhengzhou, China; Sharma B.P., Key Laboratory of Electrochemical Process and Technology of Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, China; Wang Y., Beijing University of Chinese Medicine, Beijing, China; Kassa T.S., Key Laboratory of Advanced Functional Polymer Composites, Col...
Read more