Phytochemical investigation and in vitro antioxidant activity of different leaf extracts of Salix mucronata thunb.
Bibliographic Information
Authors: El-Sayed M.M.; El-Hashash M.M.; Mohamed H.R.; Abdel-Lateef E.E.-S.
Journal: Journal of Applied Pharmaceutical Science
Publisher: Open Science Publishers LLP Inc.
Publication Date: 2015
Volume / Issue: Volume 5 / Issue 12
Pages: 80–85
ISSN: 22313354
Scopus: View on Scopus
Document Type: Article
Access: All Open Access; Hybrid Gold Open Access
Authors and Affiliations
El-Sayed M.M., Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Korniash El-Nile, Warrak El-Hadar, Giza, 12411, Egypt; El-Hashash M.M., Department of Chemistry, Faculty of Science, Ain-Shams University, El-Khalifa El-Mamoun, Abasia, Cairo, 11566, Egypt; Mohamed H.R., Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Korniash El-Nile, Warrak El-Hadar, Giza, 12411, Egypt; Abdel-Lateef E.E.-S., Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Korniash El-Nile, Warrak El-Hadar, Giza, 12411, Egypt
Abstract
The present study was aimed to determine the phytochemical constituents of Salix mucronata Thunb. leaf extracts and their antioxidant activities. Dried leaf powder was extracted with MeOH, MeOH (85%), MeOH(70%) and distilled water. The different extracts were monitored for phytochemical screening. Total phenolic and flavonoid contents were measured by Folin-Ciocalteu and aluminum chloride assays. The antioxidant potential of tested extracts was evaluated using DPPH, ABTS and total antioxidant capacity (TAC) assays. The results showed that, MeOH (85%) extract exhibited high total phenolic and flavonoid contents (TPC=131.39±2.49 mgGAE /g ext. and TFC= 67.69±1.47 mg RE /g ext.). Also, MeOH (85%) extract showed high antioxidant activities (DPPH SC50= 98.76±0.46 (μg/ml), ABTS= 45.83±0.32 mm Trolox® eq. /100 gm extract and TAC= 199.18±2.19mg equivalent of ascorbic acid /g ext.). On other hand, EtOAc fraction derived from MeOH (85%) extract exhibited the highest antioxidant activity; DPPH SC50= 50.19±0.24 (μg/ml), ABTS= 76.22±1.61(mm Trolox® eq. /100 gm ext.) and TAC= 249.86±3.74 (mg equivalent of ascorbic acid /g ext.). This study demonstrated that, S. mucronata leaf is a good source of natural antioxidants. Also, there is a high correlation between the total phenolic content and the antioxidant activity. © 2015 Mortada Mohamed El-Sayed et al.
Keywords
Antioxidant; Phytochemical screening; Salix mucronata; Total phenolic and flavonoid contents
Citation Information
Scopus Citations: 27
For comprehensive information about the Theodor Bilharz Research Institute (TBRI), its institutional activities, scientific and research achievements, clinical and hospital services, and the diverse expertise offered through its 22 specialized research and clinical departments, as well as opportunities for professional training, specialized workshops, and scientific conferences, readers are invited to visit the Institute’s official website.
The website provides regularly updated information on the Institute’s latest news, research activities, scientific initiatives, clinical services, institutional programs, and academic and professional opportunities.
English Website: https://www.tbri.sci.eg/en/
Arabic Website: https://www.tbri.sci.eg/ar/
Prepared and Uploaded by:
Abdalla F. Abdalla
Electronic Portal Unit
