Phytochemical investigation and cytotoxic characterization of bioactive constituents from Conyza dioscoridis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hamed M.M.; Mohamed M.A.; Ibrahim M.T.

Journal: International Journal of Pharmacognosy and Phytochemical Research

Publisher: International Journal of Pharmacognosy and Phytochemical Research

Publication Date: 2015

Volume / Issue: Volume 7 / Issue 5

Pages: 948–955

ISSN: 9754873

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Hamed M.M., Medicinal chemistry department, Theodor Bilharz Research Institute, Giza, Egypt; Mohamed M.A., Medicinal chemistry department, Theodor Bilharz Research Institute, Giza, Egypt; Ibrahim M.T., Al-Azhar University, Cairo, Egypt

Abstract

A follow-up chemical investigation to methanol extract of Conyza dioscoridis (Family Asteraceae) led to the isolation of seven compounds (1-7). The isolated compounds identified as 2-(3,4-dihydroxyphenyl)ethyl-2-O-[6-deoxy-α-L-mannopyranosyl-4-(3,4-dihydroxyphenyl)-2-propenoate]-β-D-glucopyranoside (1), rutin (2), isoquercetin (3), E-caffeic acid (4), gallic acid (5), quercetin (6), β-Sitosterol-3-O-β-D-glucpyranoside (7). The methanol and chloroform extracts of the plant showed cytotoxic activity against brine shrimp in a preliminary assay and inhibitory activity against colon carcinoma cells (HCT-116) with LC50 value 20, 32 μg/ml and IC50 value 25, 35.3 μg/ml, respectively. Brine shrimp lethality test was conducted on the seven isolated compounds at six different concentrations 400, 200, 100, 20, 10 and 5 μg/ml; compounds 1, 2, 3, 5, 6 and 7 showed significant cytotoxicity with LC50 value 9, 10, 11, 22, 8 and 19 μg/ml, respectively. Compounds 1, 2, 3 and 6 exhibited inhibitory activity against colon carcinoma cells with IC50 value 25, 30, 34.2 and 10 μg/ml, respectively. These results indicate that; Conyza dioscoridis has biochemical activity as potential pharmaceuticals. The chemical structures of active constituents were unambiguously determined by analysis of1HNMR,13CNMR, ESI-MS, as well as by comparison with literature data and physical methods. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.

Keywords

Bioactive constituents; Brine shrimp; Conyza dioscoridis; HCT-116; Methanol extract; Spectroscopic data; 2 (3,4 dihydroxyphenyl)ethyl 2 o [6 deoxy alpha mannopyranosyl 4 (3,4 dihydroxyphenyl) 2 propenoate]beta dextro glucopyranoside; antineoplastic agent; caffeic acid; chemical compound; Conyza dioscoridis extract; gallic acid; isoquercetin; plant extract; quercetin; rutoside; sitogluside; unclassified drug; antineoplastic activity; Artemia; Article; cancer inhibition; controlled study; Conyza; drug cytotoxicity; drug determination; drug effect; drug mechanism; drug response; drug structure; IC50; LC50; nonhuman; phytochemistry

Citation Information

Scopus Citations: 7

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