Novel IMB-ELISA assay for rapid diagnosis of human toxoplasmosis using SAG1 antigen

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hegazy S.; Farid A.; Rabae I.; El-Amir A.

Journal: Japanese Journal of Infectious Diseases

Publisher: National Institute of Health

Publication Date: 2015

Volume / Issue: Volume 68 / Issue 6

Pages: 474–480

ISSN: 13446304

DOI: 10.7883/yoken.JJID.2014.444

Scopus: View on Scopus

PubMed: 25866114

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Hegazy S., Parasitology Department, TBRI, Giza, Egypt; Farid A., Zoology Department, Cairo University, Giza, Egypt; Rabae I., Parasitology Department, TBRI, Giza, Egypt; El-Amir A., Zoology Department, Cairo University, Giza, Egypt

Abstract

Nanotechnology is important for medical diagnosis. Various nanoparticles have presented tremendous potential for diagnosing disease markers, pre-cancerous cells, fragment of viruses, specific proteins, antibodies, and other disease indicators. In general, nanoparticles are smaller than 1,000 nm and produced from different materials in different shapes such as spheres, rods, wires, and tubes. Our study aimed to develop a novel antigen-capture immunoassay based on IgG polyclonal anti-body-coated magnetic microbead nanoparticles for the rapid detection of circulating surface antigen 1 of Toxoplasma gondii in human serum samples. Sandwich ELISA elicited a sensitivity of 92%, a specificity of 92.7%, a positive predictive value (PPV) of 92%, and a negative predictive value (NPV) of 92.7%. Immunomagnetic bead-ELISA showed sensitivity (98%), specificity (96.4%), PPV (96%), and NPV (98.1%) higher than that of sandwich ELISA. It is obvious that the use of magnetic microbead nanoparticles offers the potential advantage of improving the diagnostic testing of toxoplasmosis. © 2015, National Institute of Health. All rights reserved.

Keywords

Animals; Antibodies, Protozoan; Antigens, Protozoan; Diagnostic Tests, Routine; Enzyme-Linked Immunosorbent Assay; Humans; Nanoparticles; Predictive Value of Tests; Protozoan Proteins; Rabbits; Sensitivity and Specificity; Time Factors; Toxoplasma; Toxoplasmosis; 2 diethylaminoethanol; immunoglobulin G antibody; parasite antigen; polyclonal antibody; sephadex; Toxoplasma gondii surface antigen 1; unclassified drug; nanoparticle; protozoal protein; protozoon antibody; SAG1 antigen, Toxoplasma; animal experiment; antibody production; antigen purification; Article; controlled study; double antibody sandwich enzyme linked immunosorbent assay; enzyme linked immunosorbent assay; human; immunomagnetic bead enzyme linked immunosorbent assay; ion exchange chromatography; major clinical study; nanotechnology; nonhuman; predictive value; protein analysis; rabbit; tachyzoite; Toxoplasma gondii; animal; blood; diagnostic test; isolation and purification; procedures; time factor

Citation Information

Scopus Citations: 16

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