Identification of the chemical composition of the methanolic extract of Salix tetrasperma Roxb. using LC-ESI-MS and evaluation its potential as antioxidant agent

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Wakil E.A.; Abdel-Hameed E.S.; El-Sayed M.M.; Abdel-Lateef E.E.

Journal: Der Pharma Chemica

Publisher: Scholars Research Library

Publication Date: 2015

Volume / Issue: Volume 7 / Issue 2

Pages: 168–177

ISSN: 0975413X

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

El-Wakil E.A., Laboratory of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt; Abdel-Hameed E.S., Laboratory of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt, Natural Products Analysis Laboratory, Faculty of Science, Taif University, Saudi Arabia, Chemistry Department, Faculty of Science, Taif University, Saudi Arabia; El-Sayed M.M., Laboratory of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt; Abdel-Lateef E.E., Laboratory of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt

Abstract

Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. A variety of free radical scavenging antioxidants exist within the body which many of them are derived from dietary sources like fruits and vegetables. In the present work, the total phenolic and flavonoid contents of the defatted methanolic extract of Salix tetraspermaRoxb. growing in Egypt and certain fractions (dichloromethane, ethyl acetate, n-butanol and water) derived from it was determined. The ethyl acetate fraction had high phenolic and flavonoid contents (285.48 ± 2.07 mg gallic acid equivalent /g extract and 136.13 ± 2.47 mg rutin equivalent /g extract) respectively. The antioxidant activities of these extracts were evaluated using three tests; 1,1-diphenyl picrylhydrazyl free radical scavenging activity (DPPH) method, total antioxidant capacity (TAC) assay and Azino-bis (3-ethylbenzothiazoline -6-sulfonic acid) (ABTS) assay. The results showed that although all extracts have antioxidant activity but the ethyl acetate fraction is the highest one (SC50 = 42.59 ± 0.38 mg/ml for DPPH method; 298.03 ± 6.85 mg ascorbic equivalent/g extract for TAC assay and 925.79± 4.74 mmolTrolox® equivalent / 100 g extract for ABTS assay). The identification of the chemical constituent of the defatted methanolic extract of S. tetrasperma has been performed by HPLC-ESI-MS technique. It was appeared that the chemical constituent of this extract is composed of a mixture of flavonoid and phenolic acid derivatives.

Keywords

Antioxidant activity; HPLC-ESI-MS; Phenolic and flavonoid contents; Plant extract; Salix tetrasperma; acetic acid ethyl ester; antioxidant; apigenin; ascorbic acid; butanol; caffeic acid; catechin; cinnamic acid derivative; dichloromethane; ferulic acid; flavonoid; gallic acid; hydroxybenzoic acid derivative; kaempferol 3 o alpha levo rhamnopyranoside; methanol; myrcetin; para coumaric acid; phenol derivative; protocatechuic acid; quercetin; quercetin 3 o alpha levo rhamnopyranoside; quercetin 3 o beta dextro glucopyranoside; quercetin 3 o beta dextro glucopyranoside(1-6)gallic acid; rutoside; Salix tetrasperma extract; taxifolin; trolox C; unclassified drug; unindexed drug; water; ABTS radical scavenging assay; antioxidant assay; Article; chemical composition; controlled study; DPPH radical scavenging assay; drug identification; drug isolation; Egypt; electrospray mass spectrometry; high performance liquid chromatography; willow

Citation Information

Scopus Citations: 18

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