Genomic instability in complicated and uncomplicated Egyptian schistosomiasis haematobium patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abd El-Aal A.A.; Bayoumy I.R.; Basyoni M.M.A.; Abd El-Aal A.A.; Emran A.M.; Abd El-Tawab M.S.; Badawi M.A.; Zalat R.M.; Diab T.M.

Journal: Molecular Cytogenetics

Publisher: BioMed Central Ltd.

Publication Date: 22 January 2015

Volume / Issue: Volume 8 / Issue 1

Article No.: 1

ISSN: 17558166

DOI: 10.1186/s13039-014-0104-5

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abd El-Aal A.A., Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Bayoumy I.R., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; Basyoni M.M.A., Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Abd El-Aal A.A., Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Emran A.M., Urosurgery Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Abd El-Tawab M.S., Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Badawi M.A., Pathology Department, National Research Center, Giza, Egypt; Zalat R.M., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; Diab T.M., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Background: Exploration of genetic changes during active Schistosoma infection is important for anticipation and prevention of chronic sequelae. This study aimed to explore the genomic instability in chromosomal and cellular kinetics in Egyptians suffering from uncomplicated active schistosomiasis haematobium infection in addition to chronic schistosomiasis haematobium cases complicated by bilharzial-associated bladder cancer (BAC). Results: This study was conducted on 46 schistosomiasis haemotobium cases, 22 were active (Viable S. haematobium eggs in urine samples as detected by microscopy) and 24 were chronic complicated with bladder cancer. Three cytogenetic techniques were applied; the first was quantitative nuclear-morphocytometry by means of which the Feulgen-stained nuclei were analyzed for parameters including shape, size, integrated optical-density and nuclear area. The second was Fluorescent In-Situ Hybridization (FISH) for specific p53gene-locus of chromosome 17 and the third technique was karyotyping. Concerning chronic complicated cases, the mean∈±∈SD of DNA-content in urinary bladder tissue sections was 3.18∈±∈0.65. Five samples (20.83%) of bladder tissue sections of chronic complicated cases showed diploid nuclei, 6 urinary bladder tissue samples (25%) were tetraploid, while 13 bladder samples (54.16%) were aneuploid. Epithelial cells of urine samples demonstrated aneuploidy (mean∈±∈SD∈=∈3.74∈±∈0.36).Nuclear contents showed high proliferative DNA index in all urinary epithelial cells. In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2∈±∈0.16SD. Half of these diploid smears had a high proliferation index. The difference between nuclear DNA-contents in acute and chronic cases was significant (P∈=∈0.0001). FISH technique for specific p53gene-locus and karyotyping were done on urinary bladder tissue specimens and peripheral blood monocytes of 8 chronic cases respectively. Three samples (37.5%) with invasive BAC had a deletion of the p53 gene. Karyotyping showed three cases out of the 8 chronic schistosomiasis haematobium patients with chromosomal fragmentations. Conclusions: DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues. It is an important prognostic factor in established schistosomiasis haematobium induced bladder malignancy. It has the great advantage of being applicable on urine cells making it suitable for the prediction of a tendency towards genetic instability in active schistosomiasis haematobium patients. © 2015 Abdel Aal et al.; licensee BioMed Central.

Keywords

Chromosomal abnormalities; FISH; Karyotyping; Morphocytometry; Schistosomiasis haematobium; Schistosoma; Schistosoma haematobium; adult; aged; Article; bladder cancer; bladder mucosa; child; chromosome 17; clinical article; DNA content; Egyptian; epithelium cell; female; fluorescence in situ hybridization; gene locus; genomic instability; human; human cell; human tissue; male; monocyte; parasite viability; ploidy; priority journal; schistosomiasis haematobia; tumor suppressor gene

Citation Information

Scopus Citations: 15

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