Evaluation of a method for induction of praziquantel resistance in Schistosoma mansoni

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Lotfy W.M.; Hishmat M.G.; El Nashar A.S.; Abu El Einin H.M.

Journal: Pharmaceutical Biology

Publisher: Informa Healthcare

Publication Date: 22 January 2015

Volume / Issue: Volume 53 / Issue 8

Pages: 1214–1219

ISSN: 13880209

DOI: 10.3109/13880209.2014.970289

Scopus: View on Scopus

PubMed: 25609146

Document Type: Article

Access: All Open Access; Bronze Open Access

Authors and Affiliations

Lotfy W.M., Department of Parasitology, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, Egypt; Hishmat M.G., Department of Parasitology, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, Egypt; El Nashar A.S., Department of Parasitology, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, Egypt; Abu El Einin H.M., Department of Environmental Researches and Medical Malacology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt

Abstract

Context: Praziquantel (PZQ) is a highly efficacious anthelmintic against many flatworms including schistosomes. PZQ has been in use for more than 25 years, and concern is increasing that resistance has emerged in human schistosomes in Egypt and other endemic countries. Objective: The current study was designed to evaluate a recently described method for induction of PZQ resistance in Schistosoma mansoni. Materials and methods: Successive subcurative drug treatments of Biomphalaria alexandrina snails infected with an Egyptian strain of S. mansoni were undertaken. Cercariae shed from snails exposed and unexposed to PZQ were used to infect mice. Forty-five days after infection, mice were treated with a single oral dose of PZQ in 2% aqueous solution of Cremophor-EL®. The concentration of PZQ was 0, 200, 400, or 800 mg/kg. Thirty-three days after treatment, all groups of mice were dissected to collect the S. mansoni worms by the perfusion technique. In addition, the oogram pattern was examined to study the production, maturity, and death of S. mansoni eggs in the different groups of mice. Results: The present study has shown that the sublethal dose for induction of PZQ resistance in the intra-molluscan S. mansoni stages was 500 mg/kg. The worm count and the percentage of immature eggs in different groups of mice were significantly affected by the intra-molluscan exposure to PZQ and the drug concentration used to treat infected mice. Discussion and conclusion: The results obtained herein confirm the possibility of using successive drug treatments of infected B. alexandrina to induce PZO resistance in S. mansoni. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Keywords

Antischistosomal; Biomphalaria alexandrina; Drug; Intra-molluscan; Sub-curative; Animals; Anthelmintics; Drug Evaluation, Preclinical; Drug Resistance; Mice; Praziquantel; Schistosoma mansoni; Schistosomiasis mansoni; Snails; Gastropoda; Mus; Platyhelminthes; anthelmintic agent; animal experiment; animal model; Article; cercaria; controlled study; death; dose response; drug effect; drug response; drug screening; mouse; nonhuman; parasite immunity; single drug dose; survival rate; survival time; worm egg; animal; drug effects; mortality; parasitology; preclinical study; procedures; snail

Citation Information

Scopus Citations: 18

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