Intrasplenic transplanted adult rat isolated hepatocyte fraction but not cholangiocytes forms bile canaliculi

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Olszewski W.L.; Charysz A.; Gewartowska M.; Nagui M.E.

Journal: Transplantation Proceedings

Publisher: Elsevier Inc.

Publication Date: October 2014

Volume / Issue: Volume 46 / Issue 8

Pages: 2894–2896

ISSN: 411345

DOI: 10.1016/j.transproceed.2014.09.067

Scopus: View on Scopus

PubMed: 25380945

Document Type: Conference paper

Authors and Affiliations

Olszewski W.L., Department of Surgical Research Transplantation and Epigenetics, Medical Research Center, Polish Academy of Science, 5 Pawi nskiego Str., Warsaw, 02-106, Poland, Department of Transplantation Surgery, Central Clinical Hospital, Ministry of Internal Affairs, Warsaw, Poland; Charysz A., Department of Surgical Research Transplantation and Epigenetics, Medical Research Center, Polish Academy of Science, 5 Pawi nskiego Str., Warsaw, 02-106, Poland; Gewartowska M., Department of Surgical Research Transplantation and Epigenetics, Medical Research Center, Polish Academy of Science, 5 Pawi nskiego Str., Warsaw, 02-106, Poland; Nagui M.E., Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Background. Hepatocyte transplantation (HT) has been performed in patients with liver-based metabolic disease and acute liver failure as a potential alternative to liver transplantation in countries in which ethical regulations do not allow organ transplantation. One of the problems remains that substances normally secreted by the surviving hepatocytes to bile cannot be removed because of lack of bile canaliculi. We found that ligation of the recipient's common bile duct in hepatocyte transplantation recipients is followed by formation of bile canaliculi. The question arose as to whether the signal released from the obstructed bile vasculature activated the transplanted hepatocytes (HC) or cholangiocyte (CH) to form bile canaliculi.; Methods. We transplanted separately isolated autologous HC and CH to spleens and observed the structural organization of the grafted cells.; Results. HC formed glycogen-rich clusters but not cords usually not attached to the CH of the new bile canaliculi. Separate clusters of bile canaliculi with keratin 7 and 19-positive and gamma-glutamyl transpeptidase-positive cells were observed. Transplanted CH remained keratin 7 and 19-positive and gamma-glutamyl transpeptidase positive but did not form canaliculi.; Conclusions. The transplanted HC fraction may contain hepatic progenitor cells for cholangiocytes, but they become activated only under the condition of bile stasis by an as-yet undefined factor.

Keywords

Animals; Bile Canaliculi; Common Bile Duct; Female; gamma-Glutamyltransferase; Hepatocytes; Humans; Immunohistochemistry; Ligation; Liver Failure, Acute; Liver Transplantation; Middle Aged; Rats, Inbred Lew; Spleen; cytokeratin 19; cytokeratin 7; gamma glutamyltransferase; glycogen; adult; animal experiment; cell transplantation; cell type; cholangiocyte; Conference Paper; graft recipient; hepatic progenitor cell; hepatocyte transplantation; intrahepatic bile duct; nonhuman; rat; acute liver failure; animal; human; Lewis rat; liver cell; metabolism; surgery; transplantation

Citation Information

Scopus Citations: 2

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