Hepatoprotective and antioxidant activity of Melaleuca styphelioides on carbon tetrachloride-induced hepatotoxicity in mice

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Al-Sayed E.; El-Lakkany N.M.; Seif El-Din S.H.; Sabra A.-N.A.; Hammam O.A.

Journal: Pharmaceutical Biology

Publisher: Informa Healthcare

Publication Date: 22 September 2014

Volume / Issue: Volume 52 / Issue 12

Pages: 1581–1590

ISSN: 13880209

DOI: 10.3109/13880209.2014.908398

Scopus: View on Scopus

PubMed: 25243881

Document Type: Article

Access: All Open Access; Bronze Open Access

Authors and Affiliations

Al-Sayed E., Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, Egypt; El-Lakkany N.M., Department of Pharmacology, Theodor Bilharz Research Institute, Giza, Egypt; Seif El-Din S.H., Department of Pharmacology, Theodor Bilharz Research Institute, Giza, Egypt; Sabra A.-N.A., Department of Pharmacology, Theodor Bilharz Research Institute, Giza, Egypt; Hammam O.A., Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Context: Liver disease is a serious problem. Polyphenolic compounds have marked antioxidant effect and can prevent the liver damage caused by free radicals. In vitro studies have revealed the strong antioxidant activity of an ellagitannin-rich plant, namely, Melaleuca styphelioides Sm. (Myrtaceae). Objective: In view of the limited therapeutic options available for the treatment of liver diseases, the hepatoprotective potential of the methanol extract of M. styphelioides leaves (MSE) was investigated against CCl4-induced liver injury in mice. Materials and methods: MSE was administered (500 and 1000mg/kg/d p.o.) along with CCl4 for 6 weeks. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum. Glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. The bioactive components of MSE were identified by NMR, UV and HRESI-MS/MS data. Results: MSE treatment (500 and 1000mg/kg/d) markedly inhibited the CCl4-induced increase in the levels of AST (31 and 38%), ALT (29 and 32%), ALP (13 and 19%), and MDA (22 and 37%) at the tested doses, respectively. MSE treatment markedly increased the levels of GSH (29 and 57%) and antioxidant enzymes compared with the CCl4-treated group. MSE was more effective than silymarin in restoring the liver architecture and reducing the fatty changes, central vein congestion, Kupffer cell hyperplasia, inflammatory infiltration, and necrosis induced by CCl4. The LD50 of MSE was more than 5000mg/kg. Conclusion: MSE confers potent antioxidant and hepatoprotective effects against CCl4-induced toxicity. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.

Keywords

Ellagitannins; Glutathione; Lipid peroxidation; Liver enzymes; Superoxide dismutase; Animals; Antioxidants; Carbon Tetrachloride; Disease Models, Animal; Dose-Response Relationship, Drug; Lethal Dose 50; Liver Diseases; Magnetic Resonance Spectroscopy; Male; Melaleuca; Mice; Plant Extracts; Plant Leaves; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Tandem Mass Spectrometry; 1,2,3,4,6 penta o galloyl beta dextro glucopyranose; 1,2,3,6 tetra o galloyl beta dextro glucopyranose; alanine aminotransferase; alkaline phosphatase; antioxidant; ascorbic acid; aspartate aminotransferase; gallic acid; glutathione peroxidase; glutathione reductase; glutathione transferase; kaempferol 3 o alpha levo rhamnopyranoside; liver protective agent; malonaldehyde; Melaleuca styphelioides extract; pedunculagin; plant extract; plant medicinal product; pterocarinin A; quercetin; silymarin; tellimagrandin I; unclassified drug; alanine aminotransferase blood level; alkaline phosphatase blood level; animal experiment; animal model; antioxidant activity; Article; aspartate aminotransferase blood level; controlled study; drug identification; drug mechanism; drug structure; histopathology; liver injury; liver protection; liver toxicity; Melaleuca styphelioides; mouse; nonhuman; nuclear magnetic resonance; animal; chemistry; disease model; dose response; electrospray mass spectrometry; LD50; nuclear magnetic resonance spectroscopy; plant leaf; ultraviolet spectrophotometry

Citation Information

Scopus Citations: 26

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