Evaluation of purified 27.5 kDa protoscolex antigen-based ELISA for the detection of circulating antigens and antibodies in sheep and human hydatidosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Bauomi I.R.; El-Amir A.M.; Fahmy A.M.; Zalat R.S.; Diab T.M.

Journal: Journal of Helminthology

Publisher: Cambridge University Press

Publication Date: 9 July 2014

Volume / Issue: Volume 89 / Issue 5

Pages: 577–583

ISSN: 0022149X

DOI: 10.1017/S0022149X14000479

Scopus: View on Scopus

PubMed: 25006882

Document Type: Article

Authors and Affiliations

Bauomi I.R., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; El-Amir A.M., Immunology Department, Faculty of Science, Cairo University, Egypt; Fahmy A.M., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; Zalat R.S., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; Diab T.M., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Hydatidosis is a zoonotic disease caused by the larval stage of Echinococcus granulosus, and the diagnosis of hydatidosis to date remains unresolved despite the development of many serological techniques. The present study aimed to develop an antigen-based enzyme-linked immunosorbent assay (ELISA) using IgG anti-27.5 kDa protoscolex antigen (27.5 PA) for measuring circulating protoscolex antigen (CPA), for comparison with an antibody detection assay, in sera of naturally infected sheep and humans in highly endemic areas in Egypt. In sheep, the sensitivity of ELISA in detecting anti-27.5 PA IgG and CPA was 75.0 and 60.0%, respectively, and the recorded specificity was 80.0 and 88.0%, respectively. In humans, the sensitivity of ELISA in detecting anti-27.5 PA IgG and CPA was 62.5 and 52.5%, respectively, while the specificity of the assay was 66.7 and 75.0%, respectively. In conclusion, an antibody detection assay is still superior and is more sensitive than an antigen detection assay, especially in diagnosing an active infection in which hydatid cysts are predominant. An antigen detection assay may be a useful approach to assessment of the efficacy of treatment, especially after removal of the cyst. Further studies are recommended to improve the diagnostic efficacy of an antigen-based ELISA method by using a highly purified recombinant antigen. © Cambridge University Press 2014.

Keywords

Animals; Antibodies, Helminth; Antigens, Helminth; Echinococcosis; Egypt; Enzyme-Linked Immunosorbent Assay; Humans; Sensitivity and Specificity; Sheep; Sheep Diseases; Echinococcus granulosus; Ovis aries; 27.5 kDa protoscolex antigen; circulating protoscolex antigen; immunoglobulin G; polyclonal antibody; recombinant antigen; unclassified drug; helminth antibody; parasite antigen; animal experiment; animal model; antibody detection; antigen detection; Article; Ascaris; clinical article; controlled study; cross reaction; diagnostic test accuracy study; diagnostic value; enzyme linked immunosorbent assay; Fasciola gigantica; human; intermediate host; liver hydatid cyst; nonhuman; ovine model; polyacrylamide gel electrophoresis; predictive value; Strongyloides; animal; blood; evaluation study; parasitology; procedures; veterinary

Citation Information

Scopus Citations: 13

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