Transplant of hepatocytes, undifferentiated mesenchymal stem cells, and in vitro hepatocyte-differentiated mesenchymal stem cells in a chronic liver failure experimental model: A comparative study
Bibliographic Information
Authors: El Baz H.; Demerdash Z.; Kamel M.; Atta S.; Salah F.; Hassan S.; Hammam O.; Khalil H.; Meshaal S.; Raafat I.
Journal: Experimental and Clinical Transplantation
Publisher: Baskent University
Publication Date: February 2018
Volume / Issue: Volume 16 / Issue 1
Pages: 81–89
ISSN: 13040855
Scopus: View on Scopus
PubMed: 28585911
Document Type: Article
Authors and Affiliations
El Baz H., Immunology Department, Imbaba, Giza, Egypt; Demerdash Z., Immunology Department, Imbaba, Giza, Egypt; Kamel M., Immunology Department, Imbaba, Giza, Egypt; Atta S., Immunology Department, Imbaba, Giza, Egypt; Salah F., Immunology Department, Imbaba, Giza, Egypt; Hassan S., Immunology Department, Imbaba, Giza, Egypt; Hammam O., Pathology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Khalil H., Pathology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Meshaal S., Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Raafat I., Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Objectives: Liver transplant is the cornerstone line of treatment for chronic liver diseases; however, the long list of complications and obstacles stand against this operation. Searching for new modalities for treatment of chronic liver illness is a must. In the present research, we aimed to compare the effects of transplant of undifferentiated human mesenchymal stem cells, in vitro differentiated mesenchymal stem cells, and adult hepatocytes in an experimental model of chronic liver failure. Materials and Methods: Undifferentiated human cord blood mesenchymal stem cells were isolated, pro-pagated, and characterized by morphology, gene expression analysis, and flow cytometry of surface markers and in vitro differentiated into hepatocyte-like cells. Rat hepatocytes were isolated by double perfusion technique. An animal model of chronic liver failure was developed, and undifferentiated human cord blood mesenchymal stem cells, in vitro hepato-genically differentiated mesenchymal stem cells, or freshly isolated rat hepatocytes were transplanted into a CCL4 cirrhotic experimental model. Animals were killed 3 months after transplant, and liver functions and histopathology were assessed. Results: Compared with the cirrhotic control group, the 3 cell-treated groups showed improved alanine aminotransferase, aspartate aminotransferase, albumin, and bilirubin levels, with best results shown in the hepatocyte-treated group. Histopathologic examination of the treated groups showed improved fibrosis, with best results obtained in the undifferentiated mesenchymal stem cell-treated group. Conclusions: Both adult hepatocytes and cord blood mesenchymal stem cells proved to be promising candidates for cell-based therapy in liver regeneration on an experimental level. Improved liver function was evident in the hepatocyte-treated group, and fibrosis control was more evident in the undifferentiated mesenchymal stem cell-treated group. © Başkent University 2018 Printed in Turkey. All Rights Reserved.
Keywords
Cell-mediated therapy; End-stage liver disease; Animals; Biomarkers; Carbon Tetrachloride; Cell Differentiation; Cells, Cultured; Chemical and Drug Induced Liver Injury; Cord Blood Stem Cell Transplantation; End Stage Liver Disease; Hepatocytes; Humans; Liver Cirrhosis, Experimental; Liver Regeneration; Liver Transplantation; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Mice, Inbred BALB C; Phenotype; Rats, Inbred Lew; Recovery of Function; Time Factors; alanine aminotransferase; albumin; aspartate aminotransferase; bilirubin; biological marker; adult; animal cell; animal experiment; animal model; animal tissue; Article; carbon tetrachloride-induced liver cirrhosis; cell isolation; cell structure; cell transplantation; chronic liver failure; comparative study; controlled study; female; flow cytometry; gene expression assay; histopathology; human; human cell; infant; liver cell; liver function; mesenchymal stem cell; nonhuman; rat; animal; Bagg albino mouse; cell culture; chemically induced; convalescence; experimental liver cirrhosis; Lewis rat; mesenchymal stroma cell; metabolism; pathology; pathophysiology; procedures; time factor; toxic hepatitis; transplantation
Citation Information
Scopus Citations: 20
For comprehensive information about the Theodor Bilharz Research Institute (TBRI), its institutional activities, scientific and research achievements, clinical and hospital services, and the diverse expertise offered through its 22 specialized research and clinical departments, as well as opportunities for professional training, specialized workshops, and scientific conferences, readers are invited to visit the Institute’s official website.
The website provides regularly updated information on the Institute’s latest news, research activities, scientific initiatives, clinical services, institutional programs, and academic and professional opportunities.
English Website: https://www.tbri.sci.eg/en/
Arabic Website: https://www.tbri.sci.eg/ar/
Prepared and Uploaded by:
Abdalla F. Abdalla
Electronic Portal Unit
