Role of microRNA-183 based theranostics through targeting TPM1 in bladder cancer

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mamdouh S.; Aboushousha T.; Hemida E.; El-Araby R.E.; Elesaily K.; Hammad G.; Magdy M.

Journal: Discover Oncology

Publisher: Springer Science and Business Media B.V.

Publication Date: 13 November 2025

Volume / Issue: Volume 16 / Issue 1

Article No.: 2100

ISSN: 27306011

DOI: 10.1007/s12672-025-03829-w

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Mamdouh S., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, Egypt; Aboushousha T., Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hemida E., Biochemistry Department, Obstetrics and Gynecology Hospital, Faculty of Science, Ain Shams University, Cairo, Egypt; El-Araby R.E., Department of Basic and Clinical Translation Sciences, Tufts University School of Dental Medicine, Boston, 02111, MA, United States, Central Lab, Theodor Bilharz Research Institute, Giza, Egypt; Elesaily K., Urology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hammad G., Faculty of Biotechnology, October University for Modern Sciences and Arts, Giza, Egypt; Magdy M., Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Background: MicroRNA-183 (miR-183-5p), a noncoding RNA, is upregulated in bladder carcinoma (BC). Although it has been implicated in oncogenesis, its precise regulatory effects and biological functions remain unclear. Tropomyosin-1 (TPM1) was shown to be downregulated in solid tumors and was previously identified as a novel tumor suppressor gene. Objectives: Our study focuses on the prognostic, diagnostic, and therapeutic potential of miR-183-5p in bladder carcinoma and assess TPM1 gene targets and their modulatory functions. Methods: Urine cytology, cystectomy and transurethral resection (TUR) biopsies from 148 BC patients were collected. TPM1 protein and miR-183-5p expressions were assessed through immunohistochemistry (IHC) and real-time PCR respectively. In vitro assay investigated the effect of miR-183-5p on TPM1mRNA in bladder carcinoma cell lines, then confirmed by comparing miR-183-5p mimics in non-cancerous and urothelial carcinoma cell lines. Concomitant TPM1 gene expression was examined, and the theranostic miR-183-5p potential was evaluated. Results: Upregulation of miR-183-5p expression in BC tissue biopsies and urine cytologies was noted, contrasting the downregulation of TPM1 protein expression in the high-grade, high-stage, lymph node metastatic BC tissues (in comparison to non-cancerous, low-grade, low-stage non-lymph node metastasizing BCs). Moreover, the miR-183-5p oncogenic influence was responsive in targeting TPM1 gene at 3′UTR region, and miR‑183‑5p.1 restricted TPM1 expression in T24 cells. Conclusion: This study provides the first illustration of the miR-183-5p–TPM1 axis in bladder carcinoma, supporting the theranostic role of miR-183-5p as an onco-miR in BC progression, diagnosis, and prognostication. © The Author(s) 2025.

Keywords

Biopsy; Bladder carcinomas; MicroRNA; miR-183-5p; Theranostic; TPM1; Urine cytology; alpha tropomyosin; microRNA 183; miR 183 5p; unclassified drug; Article; biotechnology; bladder cancer; bladder carcinoma; cancer staging; carcinoma cell line; controlled study; cystectomy; diagnostic test accuracy study; electronic spreadsheet; female; gene; genetic transfection; human; human cell; human tissue; immunohistochemistry; major clinical study; male; protein expression; real time polymerase chain reaction; receiver operating characteristic; SV-HUC-1 cell line; T24 cell line; theranostics; TPM1 gene; transurethral resection; transurethral resection of bladder tumor; transurethral resection of the bladder; tumor suppressor gene; urothelial carcinoma cell line

Citation Information

Scopus Citations: 0

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