Protective effect of isopimpinellin on ovalbumin-induced airway inflammation and oxidative stress in mouse model of asthma

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelmawgood I.A.; Kotb M.A.; Eltras H.S.H.; Mahana N.A.; Goda L.; Rochdi A.M.; Sayed N.H.; Elsafoury R.H.; Saber A.M.; Youssef M.N.; Waheeb N.G.; Ashry H.; Ebeed B.W.; Al-Rifai M.W.A.; Badr A.M.; Abdelfattah H.H.

Journal: Respiratory Physiology and Neurobiology

Publisher: Elsevier B.V.

Publication Date: October 2025

Volume / Issue: Volume 337

Article No.: 104470

ISSN: 15699048

DOI: 10.1016/j.resp.2025.104470

Scopus: View on Scopus

PubMed: 40614911

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Abdelmawgood I.A., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Kotb M.A., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Eltras H.S.H., Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt; Mahana N.A., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Goda L., Pathology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Rochdi A.M., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Sayed N.H., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Elsafoury R.H., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Saber A.M., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Youssef M.N., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Waheeb N.G., Biotechnology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Ashry H., Basic Sciences Department, Saint Petersburg University in Cairo, Cairo, Egypt; Ebeed B.W., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Al-Rifai M.W.A., Al-Makassed Islamic Charitable, East, Jerusalem, Biet Jala Hospital, Biet Jala, Palestine; Badr A.M., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Abdelfattah H.H., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt

Abstract

Background: Asthma is a chronic airway disease characterized by immunological dysregulation that causes sustained inflammation, leading to aberrant airway narrowing and mucus production. Isopimpinellin (IP) is a naturally occurring furanocoumarin, generated by an association of coumarin and a furan ring, possessing antifungal, antibacterial, and antioxidant properties; however, its potential in asthma treatment has not been investigated. This study sought to evaluate the anti-inflammatory and antioxidant effects of IP in an ovalbumin (OVA)-induced murine model of asthma. Methods: Mice were grouped into four groups (n = 8 per group): control, OVA, and IP-treated (35 and 70 mg/kg). At the end of the experiment, lung oxidative stress, inflammation, and histopathological changes were evaluated. Results: IP treatment significantly ameliorated airway oxidative stress by decreasing the concentration of malondialdehyde (MDA) and increasing the levels of antioxidants, including glutathione (GSH), catalase (CAT), and glutathione peroxidase (GPx); reduced inflammatory cell infiltration; mitigated histological changes; and decreased levels of IL-4, IL-13, and IgE. Additionally, IP prevented NF-κB phosphorylation. This study is the first examination of the anti-asthmatic properties of IP in allergic asthma in murine models. Conclusion: Our data indicate that IP may function as an anti-asthmatic medication via regulating oxidative stress and inflammation. © 2025 Elsevier B.V.

Keywords

Airway inflammation; Allergic asthma; Isopimpinellin; Ovalbumin; Oxidative stress; Animals; Anti-Asthmatic Agents; Anti-Inflammatory Agents; Antioxidants; Asthma; Disease Models, Animal; Female; Furocoumarins; Inflammation; Lung; Mice; Mice, Inbred BALB C; biological marker; catalase; glutathione; glutathione peroxidase; immunoglobulin E; interleukin 13; interleukin 4; malonaldehyde; transcription factor RelA; antiasthmatic agent; antiinflammatory agent; antioxidant; furocoumarin derivative; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; Article; bronchoalveolar lavage fluid; cell count; controlled study; drug megadose; histology; histopathology; immunoglobulin blood level; immunohistochemistry; inflammatory cell; inflammatory infiltrate; low drug dose; lung parenchyma; mouse; mouse model; nonhuman; ovalbumin-induced airway inflammation; protein phosphorylation; animal; Bagg albino mouse; disease model; drug effect; drug therapy; pathology

Citation Information

Scopus Citations: 2

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