Innovative therapeutic modalities of purified platelet-derived growth factors on PDX1, Neurog3, and renin expressions in STZ-induced diabetic nephropathy and pancreatic injury

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Elzallat M.; Hassan M.; Fahmy H.M.; El-Ahwany E.; Salama N.M.; Abu-Taleb H.; Aboushousha T.; Balata M.; Mohammed D.M.

Journal: Life Sciences

Publisher: Elsevier Inc.

Publication Date: October 2025

Volume / Issue: Volume 379

Article No.: 123921

ISSN: 243205

DOI: 10.1016/j.lfs.2025.123921

Scopus: View on Scopus

PubMed: 40834996

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Elzallat M., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hassan M., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Fahmy H.M., Clinical pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; El-Ahwany E., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Salama N.M., Medical Pharmacology, Faculty of Medicine, Cairo University, Egypt; Abu-Taleb H., Enviromental Research Department, Theodor Bilharz Research Institute, Giza, Egypt; Aboushousha T., Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Balata M., University Hospital Bonn, Venusberg-Campus 1, Bonn, 53127, Germany, University of Erlangen-Nuremberg, Erlangen, Germany; Mohammed D.M., Nutrition and Food Sciences Department, National Research Centre, Dokki, Giza, 12622, Egypt

Abstract

Diabetic nephropathy is the predominant etiology of advanced renal disease, and its prevalence has increased significantly over recent years. The primary management strategy focuses on controlling identified risk factors; however, this approach only slows disease progression and cannot halt or reverse it. Therefore, novel therapeutic strategies are urgently needed. Forty-eight male rats were used in this study. After the experiment, kidney and pancreas tissues were removed and maintained for histopathological examination and gene expression analysis, and blood samples were taken to evaluate biochemical markers. Nutritional parameters were also evaluated. The diabetic nephropathy + PRP group and the diabetic nephropathy + L-GFs group showed reduced blood glucose levels and renin expression, alongside increased PDX1 and Neurog3 expression compared to the diabetic nephropathy group. Additionally, these groups exhibited decreased cytokine and malondialdehyde (MDA) levels, with significant improvements in superoxide dismutase (SOD) activity, hepatic function, and renal function biomarkers. Histopathological analysis of the kidneys and pancreas from both treatment groups showed significant improvements compared to the diabetic nephropathy group. In conclusion, administering platelet-rich plasma (PRP) and lyophilized growth factors (L-GFs) represents an innovative and promising treatment strategy for diabetic nephropathy and accompanying consequences. © 2025 The Authors

Keywords

Diabetic nephropathy; Gene expression; Lyophilized GFs (L-GFs); Oxidative stress; Pancreatic injury; Platelet-rich plasma; Animals; Basic Helix-Loop-Helix Transcription Factors; Blood Glucose; Diabetes Mellitus, Experimental; Diabetic Nephropathies; Homeodomain Proteins; Intercellular Signaling Peptides and Proteins; Kidney; Male; Nerve Tissue Proteins; Pancreas; Rats; Rats, Sprague-Dawley; Renin; Streptozocin; Trans-Activators; alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; biochemical marker; biological marker; cytokine; glucose; growth factor; insulin; interleukin 6; malonaldehyde; neurogenin 3; platelet derived growth factor; superoxide dismutase; transcription factor PDX 1; tumor necrosis factor; urea; basic helix loop helix transcription factor; homeodomain protein; nerve protein; Neurog3 protein, rat; pancreatic and duodenal homeobox 1 protein; signal peptide; transactivator protein; animal experiment; animal model; animal tissue; Article; biochemical analysis; blood sampling; body weight; body weight gain; clinical feature; colorimetry; controlled study; diabetes mellitus; drug therapy; enzyme activity; enzyme linked immunosorbent assay; experimental diet; fasting blood glucose level; feed efficiency; food intake; freeze drying; gene expression profiling; glucose blood level; histopathology; hyperglycemia; inflammation; kidney function; kidney tissue; light microscopy; liver function; mortality; nonhuman; pancreas injury; pancreas islet beta cell; pancreas tissue; posterior vena cava; protein expression; rat; real time polymerase chain reaction; reverse transcription; RNA extraction; spectrophotometry; streptozotocin-induced diabetic nephropathy; subcutaneous drug administration; thrombocyte; thrombocyte rich plasma; vein puncture; Wistar rat; animal; biosynthesis; complication; drug effect; experimental diabetes mellitus; genetics; metabolism; pathology; Sprague Dawley rat; therapy

Citation Information

Scopus Citations: 0

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