Therapeutic outcome of bone marrow stem cells and ginger in chronic murine toxoplasmosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hassan Z.R.; Shaaban Y.M.; Mahmoud E.M.; Ali A.M.M.; Al-shahed F.A.-Z.N.; Salama D.E.A.; Elsokary A.N.I.; Abdelgalil R.M.; Alanany M.A.; Koullah M.T.; Seliem N.; Amin D.R.; Mohamed S.A.; Etewa M.A.M.; Mahmoud Mousa S.N.; Amin M.M.; Hamouda M.M.M.; Atta S.A.; Gadallah R.A.; Nasr S.M.

Journal: Journal of Parasitic Diseases

Publisher: Springer

Publication Date: 17 May 2025

Volume / Issue: Volume 49 / Issue 4

Pages: 1012–1021

ISSN: 9717196

DOI: 10.1007/s12639-025-01817-z

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Hassan Z.R., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Departments of Parasitology, Benha National University (BNU) Qalyubia, Obour City, Egypt; Shaaban Y.M., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Mahmoud E.M., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Ali A.M.M., Departments of Histology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Al-shahed F.A.-Z.N., Departments of Histology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Salama D.E.A., Departments of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Department of Pathology, School of Medicine, Badr University in Cairo (BUC), Cairo, Badr City, Egypt; Elsokary A.N.I., Departments of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Abdelgalil R.M., Departments of Anatomy and Embryology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Alanany M.A., Departments of Anatomy and Embryology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Koullah M.T., Departments of Anatomy and Embryology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Seliem N., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Department of Medical Biochemistry and Molecular Biology, School of Medicine, Badr University in Cairo (BUC), Cairo, Badr City, Egypt; Amin D.R., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Mohamed S.A., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Etewa M.A.M., Departments of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Mahmoud Mousa S.N., Departments of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Amin M.M., Departments of Pharmacology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Hamouda M.M.M., Departments of Pharmacology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Atta S.A., Departments of Immunology, Theodor Bilharz Research Institute, Giza, Egypt; Gadallah R.A., Departments of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Giza, Egypt; Nasr S.M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt, Department of Biochemistry and Molecular Biology, School of Biotechnology, Badr University in Cairo (BUC), Cairo, Badr City, Egypt

Abstract

Toxoplasmosis is a common protozoal disease that can cause serious complications. Hence the available drug therapies possess limited activity against chronic forms of the disease; thus, it is urgent to find more effective agents. The current study highlighted the therapeutic activity of bone marrow mesenchymal stem cells (BMSCs) and ginger combined with spiramycin against chronic experimental toxoplasmosis. A total of 48 male Swiss albino mice were distributed into 8 groups: non-infected non-treated, infected non-treated, infected treated by spiramycin, infected treated by BMSCs, infected treated by ginger, infected treated by combined BMSCs and spiramycin, infected treated by combined BMSCs and ginger, and infected treated by combined BMSCs, ginger, and spiramycin. The evaluation was performed through parasitological counting of brain cyst burden, histopathological examination, immunohistochemical cyclooxygenase-2 (COX-2) staining assessment, serum IL-10 measurement, and apoptotic gene markers assay. The results revealed that combined BMSCs, ginger, and spiramycin displayed significantly reduced parasitic cyst burden, restored histopathological changes, decreased COX2 expression, and downregulated caspases gene expression. It can be concluded that adding BMSCs and ginger to spiramycin provides a potent therapeutic agent against chronic toxoplasmosis. © Indian Society for Parasitology 2025.

Keywords

Apoptosis; Caspases; COX2; Ginger; IL-10; Spiramycin; Toxoplasmosis; 5' nucleotidase; beta1 integrin; caspase; cyclooxygenase 2 inhibitor; Hermes antigen; interleukin 10; animal cell; animal experiment; animal model; animal tissue; architecture; Article; bone marrow cell; bone marrow mesenchymal stem cell; cell infiltration; controlled study; down regulation; gene expression; hematopoietic stem cell; histopathology; immunohistochemistry; immunology; light microscopy; male; marker gene; mesenchymal stem cell; mouse; murine toxoplasmosis; nonhuman; oxidative stress; polymerase chain reaction; protozoal infection; real time polymerase chain reaction; treatment outcome

Citation Information

Scopus Citations: 0

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