Phyto-metabolomic investigation and anti-migratory potential of Moringa oleifera Lam. and Moringa peregrina (Forssk.) Fiori against hepatic carcinoma cell line

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Osman E.E.A.; Abdel-Hameed E.-S.S.; Shemis M.A.; Mamdouh S.

Journal: Acta Pharmaceutica Sciencia

Publisher: Istanbul Medipol University

Publication Date: 2025

Volume / Issue: Volume 63 / Issue 1

Pages: 152–177

ISSN: 13072080

DOI: 10.23893/1307-2080.APS6310

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Osman E.E.A., Theodor Bilharz Research Institute, Department of Medicinal Chemistry, Giza, 12411, Egypt; Abdel-Hameed E.-S.S., Theodor Bilharz Research Institute, Department of Medicinal Chemistry, Giza, 12411, Egypt; Shemis M.A., Theodor Bilharz Research Institute, Department of Biochemistry and Molecular Biology, Giza, 12411, Egypt; Mamdouh S., Theodor Bilharz Research Institute, Department of Biochemistry and Molecular Biology, Giza, 12411, Egypt

Abstract

The present study aims to characterize the active ingredients of Moringa oleif-era (MO) and Moringa peregrina (MP) leaf extracts and evaluate their cytotoxic and anti-migration efficacies against liver cancer cells (HepG2). The chemical profiling of the aqueous and methanolic extracts of both Moringa species was carried out using LC-ESI-MS led to identifying of 37 compounds classified as flavonoids, phenolic acids, glucosinolate, and phenyl ethanoid. The MTT assay was used to assess the cytotoxic effect, which revealed to, the increasing of the MO and MP extracts’ concentration adversely decreased the treated cells’ viabil-ity. Treating of HepG2 cells by MO and MP for different time intervals effectively inhibited wound closure, while the cells migratory ability in the treated cells was decreased. This study reports that MO and MP extracts exhibited promising cytotoxic effects and inhibited migration of highly metastatic HepG2 cell lines, which may be due to the presence of polyphenolic compounds. © 2025, Istanbul Medipol University. All rights reserved.

Keywords

anti-migration; HepG2; LC-ESI-MS; Moringa; phenolic compounds; apigenin; flavonoid; glucosinolate; isorhamnetin; kaempferol; lactate dehydrogenase; Moringa extract; phenol derivative; plant extract; quercetin; unclassified drug; antineoplastic activity; Article; cell growth; cell migration; cell viability; chemical structure; controlled study; density; electrospray; Hep-G2 cell line; human; human cell; IC50; liquid chromatography-mass spectrometry; liver cell carcinoma; migration inhibition; Moringa oleifera; MTT assay; particle size; plant taxonomy; room temperature; tumor growth; wound closure; wound healing

Citation Information

Scopus Citations: 1

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