Isolation and Characterization of Superoxide Dismutase from Tectus dentatus Marine Snail with Antimicrobial and Antitumor Activities

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdel-Monsef M.M.; Darwish D.A.; Helmy M.S.; Esa S.S.; Hamed A.A.; Zayed K.M.; Habib M.R.; Masoud H.M.

Journal: Asian Pacific Journal of Cancer Prevention

Publisher: Asian Pacific Organization for Cancer Prevention

Publication Date: 2025

Volume / Issue: Volume 26 / Issue 12

Pages: 4537–4548

ISSN: 15137368

DOI: 10.31557/APJCP.2025.26.12.4537

Scopus: View on Scopus

PubMed: 41459868

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abdel-Monsef M.M., Molecular Biology Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt, Proteome Research Lab, Central Laboratories Network and Centers of Excellence, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt; Darwish D.A., Molecular Biology Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt, Proteome Research Lab, Central Laboratories Network and Centers of Excellence, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt; Helmy M.S., Molecular Biology Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt, Proteome Research Lab, Central Laboratories Network and Centers of Excellence, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt; Esa S.S., Molecular Biology Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt, Proteome Research Lab, Central Laboratories Network and Centers of Excellence, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt; Hamed A.A., Microbial Chemistry Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt; Zayed K.M., Medical Malacology Department, Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza, 12411, Egypt; Habib M.R., Medical Malacology Department, Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza, 12411, Egypt; Masoud H.M., Molecular Biology Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt, Proteome Research Lab, Central Laboratories Network and Centers of Excellence, National Research Centre, 33 El-Buhouth St., Dokki, Giza, 12622, Egypt

Abstract

Objective: The aim of this study is to reports the purification, biochemical characterization, and biological activities of superoxide dismutase (SOD) isoenzymes from the marine snail Tectus dentatus (TdSOD). Methods: The enzyme was purified through DEAE-cellulose ion-exchange and Sephacryl S-300 size-exclusion chromatography, yielding two distinct isoenzymes, TdSOD1 and TdSOD2. TdSOD1 was further purified to homogeneity with a 6.9-fold purification and a specific activity of 658.3 U/mg. Results: Electrophoretic analyses confirmed the enzyme's purity and revealed a native molecular weight of approximately 180 kDa, composed of subunits around 90 kDa. TdSOD1 showed optimal activity at pH 7.8 and was strongly activated by Zn²⁺ and Cu²⁺, while inhibitors such as KCN and H₂O₂ significantly reduced its activity. Functionally, TdSOD1 demonstrated notable antimicrobial activity, especially against Candida albicans (97.3% inhibition) and Gram-negative bacteria including Pseudomonas aeruginosa (61.7%) and Escherichia coli (60.2%). Lower activity was observed against Klebsiella pneumoniae (39.1%) and Staphylococcus aureus (2.9%). In anticancer assays, TdSOD1 exerted a dose-dependent cytotoxic effect on MCF-7 and MDA-MB-231 breast cancer cell lines, with an IC₅₀ of 33.42 μg/mL for MDA-MB-231 cells. Conclusion: These results suggest that TdSOD1 possesses promising antimicrobial and anticancer potential, supporting its future exploration as a multifunctional therapeutic agent. This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.

Keywords

Antimicrobial; characterization; Marine snails; Purification; Superoxide dismutase; Tectus dentatus; Animals; Anti-Infective Agents; Antineoplastic Agents; Candida albicans; Cell Proliferation; Humans; Microbial Sensitivity Tests; Snails; antiinfective agent; antineoplastic agent; animal; chemistry; drug effect; enzymology; human; isolation and purification; microbial sensitivity test; snail

Citation Information

Scopus Citations: 5

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