Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Helal N.S.; Maher S.; Samir S.; Elmeligy H.A.; Aboul-Ezz M.A.; Aboushousha T.; Moussa M.

Journal: Journal of Cancer Research and Clinical Oncology

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 29 January 2025

Volume / Issue: Volume 151 / Issue 2

Article No.: 56

ISSN: 1715216

DOI: 10.1007/s00432-024-06055-z

Scopus: View on Scopus

PubMed: 39878802

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Helal N.S., Department of Pathology, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Maher S., Department of Immunology, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Samir S., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt; Elmeligy H.A., Department of Surgery, Theodor Bilharz Research Institute, Giza, Egypt; Aboul-Ezz M.A., Department of Hepatology and Gastroenterology, Theodor Bilharz Research Institute, Giza, Egypt; Aboushousha T., Department of Pathology, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Moussa M., Department of Pathology, Theodor Bilharz Research Institute, Giza, 12411, Egypt

Abstract

Introduction: Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. The roles of the transcription factor special AT-rich binding protein-2 (SATB2) and β-catenin in PDAC have been a subject of controversy. We aimed to assess the diagnostic and prognostic impact of SATB2 and β-catenin in PDAC. Methods: We analyzed 44 paraffin-embedded tissue blocks along with corresponding blood and pancreatic tissues. We evaluated SATB2 expression using immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA). β-catenin was assessed using IHC and real-time polymerase chain reaction (qPCR). Results: High SATB2 expression and low β-catenin expression were associated with a poor prognosis in PDAC, including advanced pathological tumor stage (pT-stage), pathological lymph node stage (pN-stage), and TNM stage. We found a positive correlation between SATB2 expression assessed by IHC and the concentration of SATB2 in both serum and tissue samples measured by ELISA. We observed a positive correlation between β-catenin expression assessed by IHC and β-catenin levels measured by qPCR. Conclusions: SATB2 and β-catenin could provide valuable insights into the development of pancreatic cancer, and targeting them may be beneficial for the prevention and treatment of PDAC. The levels of SATB2 in serum show promise for the diagnosis and tumor invasion of pancreatic cancer. © The Author(s) 2025.

Keywords

ELISA; IHC; Pancreatic ductal adenocarcinoma; qPCR; SATB2; β-catenin; Aged; beta Catenin; Biomarkers, Tumor; Carcinoma, Pancreatic Ductal; Female; Humans; Male; Matrix Attachment Region Binding Proteins; Middle Aged; Neoplasm Staging; Pancreatic Neoplasms; Prognosis; Transcription Factors; binding protein; biological marker; special at rich binding protein 2; transcription factor; unclassified drug; CTNNB1 protein, human; matrix attachment region binding protein; SATB2 protein, human; tumor marker; adult; Article; blood sampling; cancer patient; cancer prognosis; cancer staging; clinical article; controlled study; enzyme linked immunosorbent assay; histopathology; human; human tissue; immunohistochemistry; lymph node; lymph vessel metastasis; pancreas cancer; pancreas tissue; pancreatic ductal carcinoma; perineural invasion; protein expression; protein expression level; real time polymerase chain reaction; sensitivity and specificity; tissue homogenate; tumor invasion; diagnosis; genetics; metabolism; pancreas tumor; pathology

Citation Information

Scopus Citations: 3

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