Production of Antimicrobial Bioactive Compounds from Endophytic Fungi Against Escherichia coli Strain NRC1 Isolated from the African Catfish

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Sabry N.M.; Khalil W.K.B.; El-Fiky S.A.; Abdel-Gawad F.Kh.; Ghareeb M.A.; El Awady M.E.; Hamed A.A.; El Sayed G.H.

Journal: Egyptian Journal of Aquatic Biology and Fisheries

Publisher: Egyptian Society for the Development of Fisheries and Human Health

Publication Date: 1 September 2024

Volume / Issue: Volume 28 / Issue 5

Pages: 2167–2182

ISSN: 11106131

DOI: 10.21608/ejabf.2024.389800

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Sabry N.M., Water Pollution Research Department, National Research Center (NRC), Dokki, 12622, Egypt, Center of Excellence for Research and Applied Studies on Climate Change and Sustainable Development, National Research Center (NRC), 33 El Bohouth St. Dokki, Giza, 12622, Egypt; Khalil W.K.B., Center of Excellence for Research and Applied Studies on Climate Change and Sustainable Development, National Research Center (NRC), 33 El Bohouth St. Dokki, Giza, 12622, Egypt, Department of Cell Biology, National Research Center, El-Bohouth St., Dokki, Giza, 12622, Egypt; El-Fiky S.A., Department of Cell Biology, National Research Center, El-Bohouth St., Dokki, Giza, 12622, Egypt; Abdel-Gawad F.Kh., Water Pollution Research Department, National Research Center (NRC), Dokki, 12622, Egypt, Center of Excellence for Research and Applied Studies on Climate Change and Sustainable Development, National Research Center (NRC), 33 El Bohouth St. Dokki, Giza, 12622, Egypt, National Biotechnology Network of Expertise (NBNE), Academy of Scientific Research and Technology (ASRT), Cairo, 11516, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt; El Awady M.E., Microbial Biotechnology Department, Biotechnology Research Institute National Research Center, Cairo, Egypt; Hamed A.A., Microbial Chemistry Department, National Research Center, Dokki, Cairo, 12622, Egypt; El Sayed G.H., Microbial Chemistry Department, National Research Center, Dokki, Cairo, 12622, Egypt

Abstract

In the present study, Escherichia coli was successfully isolated from a catfish sample collected from the El-Moheet drain in Giza, Egypt, with a prevalence rate of 20.8%. The bacterial isolates were confirmed through biochemical tests, demonstrating characteristics consistent with E. coli, such as indole production, nitrate reduction, and acid production. The strain was further confirmed by 16S rRNA sequencing and deposited in GenBank (PQ218973.1). In parallel, five endophytic fungi were extracted from seagrasses in Wadi El-Natron, El-Beheira, Egypt, and exhibited notable antimicrobial properties. Crude extracts from these fungi, particularly the F2 isolate, showed strong antimicrobial activity, with an efficacy of 89.40 ± 2.44% against pathogenic microorganisms. The most potent endophyte was identified as Aspergillus sp. through morphological and genetic analysis, classified as Aspergillus sp. isolate NRC1, and deposited in GenBank (PQ270262.1). GC-MS analysis of the fungal extracts revealed 36 bioactive compounds, with trans-1,4-diacetoxy-2-butene being the most abundant. ADME analysis of this compound indicated favorable physicochemical properties, including high gastrointestinal absorption and blood-brain barrier permeability. It exhibited no significant inhibitory effects on major cytochrome P450 enzymes, suggesting a low risk of drug interactions. Despite potential challenges in synthetic optimization, trans-1,4-diacetoxy-2-butene shows promise for further therapeutic development. © 2024, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.

Keywords

Antimicrobial; Catfish; Endophytic fungi; Escherichia coli; Secondary metabolite

Citation Information

Scopus Citations: 0

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