Evaluation of serum MicroRNA 21, MicroRNA 192 and serum TGFβ1 in type 2 diabetes mellitus patients and their relation to diabetic nephropathy

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abou Eleila J.G.; Abdel Wahab Mohamed A.; Waked E.A.; Kamel L.N.; Shawky Amin H.; Elhanafi H.M.

Journal: Egyptian Journal of Medical Human Genetics

Publisher: Springer Nature

Publication Date: 8 July 2024

Volume / Issue: Volume 25 / Issue 1

Article No.: 80

ISSN: 11108630

DOI: 10.1186/s43042-024-00544-z

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abou Eleila J.G., Department of Clinical Chemistry, Theodor Bilharz Research Institute, Giza, Egypt; Abdel Wahab Mohamed A., Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt; Waked E.A., Department of Nephrology, Theodor Bilharz Research Institute, Giza, Egypt; Kamel L.N., Department of Clinical Chemistry, Theodor Bilharz Research Institute, Giza, Egypt; Shawky Amin H., Department of Clinical Chemistry, Theodor Bilharz Research Institute, Giza, Egypt; Elhanafi H.M., Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background: Diabetic nephropathy (DN) is a frequent and long-lasting microvascular consequence that has an established connection with diabetes. It serves as the primary etiological agent of end-stage renal disease, a critical renal disorder that develops on a worldwide level. The molecular pathophysiology of DN is multifactorial, such as transforming growth factor-beta [TGF-β] which affects the expression of miRNAs such as miRNA-21 and miRNA-192 during renal fibrosis. However, to date, the clinical application is inadequate due to discrepancies observed in the published data. This cross-sectional investigation aimed to assess the correlation between serum TGF-β1, miRNA-21 and 192, and glycemic control, metabolic abnormalities, and renal function in patients with type II diabetes. Methods: Based on the albumin/creatinine ratio (ACR), fifty subjects with type II diabetes were divided into three categories: Group I consisted of individuals with normoalbuminuria (n = 16), Group II of microalbuminuria (n = 16), and Group III of overt proteinuria (n = 18). All participants were subjected to the estimation of mature miRNA-21 and miRNA-192 by TaqMan two-step stem loop qRT-PCR and serum TGFβ1 level by ELISA. Results: There was an upregulation in miRNA-21 expression in the 3 different groups of patients (p value = 0.043). The serum fold change (FC) of miRNA-21 showed significantly greater median values in patients with overt proteinuria compared to those with normoalbuminuria (5.57 FC versus 1.11 FC, p = 0.017). A positive correlation (r = 0.343) (p = 0.013) was observed between the ACR and the median levels of miRNA-21, which was statistically significant. No statistically significant distinctions were detected in the concentrations of serum TGF-β1 or miRNA-192 among the three patient groups (p values of 0.234 and 0.225, respectively). Conclusion: The findings of the present research implied that miRNA-21 might function as an early indicator of renal pathology associated with diabetes mellitus (DM). © The Author(s) 2024.

Keywords

Diabetic nephropathy; miR192; miR21; TGF-β; complementary DNA; glucose; hemoglobin A1c; high density lipoprotein; low density lipoprotein; microRNA 192; microRNA 21; transforming growth factor beta1; triacylglycerol; adult; albumin to creatinine ratio; Article; body mass; cholesterol blood level; clinical article; controlled study; cross-sectional study; diabetic patient; end stage renal disease; enzyme linked immunosorbent assay; female; glucose blood level; glycemic control; human; kidney fibrosis; kidney function; male; microalbuminuria; middle aged; non insulin dependent diabetes mellitus; proteinuria; real time polymerase chain reaction; real time reverse transcription polymerase chain reaction; triacylglycerol blood level; upregulation

Citation Information

Scopus Citations: 9

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