Immunochromatography Lateral Flow Strip Enhancement Based on Passive Gold Nanoparticles Conjugation to Detect Schistosma haematobium Antigens in Human Serum

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Shall M.N.; Aly I.; Samen A.; Salama W.M.; Baakdah F.

Journal: Acta Parasitologica

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 16 May 2024

Volume / Issue: Volume 69 / Issue 2

Pages: 1267–1274

ISSN: 12302821

DOI: 10.1007/s11686-024-00841-y

Scopus: View on Scopus

PubMed: 38753102

Document Type: Article

Access: All Open Access; Green Open Access; Hybrid Gold Open Access

Authors and Affiliations

El-Shall M.N., Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt; Aly I., Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt; Samen A., Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt; Salama W.M., Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt; Baakdah F., Department of Medical Laboratory Science, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia, Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

Abstract

Purpose: This study aimed to develop and evaluate a lateral flow card for the detection of active Schistosoma haematobium infection. Methods: In order to prepare the immunochromatography lateral flow strip (ICLFS), antibodies purified from schistosomiasis were conjugated passively with gold nanoparticles using a potassium carbonate buffer. Results: The novel ICLFS was able to correctly identify 64 out of 67 samples of schistosomiasis, 6 out of 90 samples of other parasites, and 0 out of 27 control samples. Sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) were 95.5%, 93.3%, 90%, and 91.4% respectively. Comparatively, the sensitivity, specificity, NPV, and PPV of sandwich enzyme-linked immunosorbent assays (ELISA) conjugated with gold nanoparticles (AuNPs) were 91.1%, 88.8%, 85.9%, and 84.4% respectively. The increased sensitivity and specificity of ICLFS produced superior results to those of sandwich ELISA. Conclusion: In conclusion, ICLFS is more beneficial and precise than sandwich ELISA for detection of S. haematobium infection at early stage. © The Author(s) 2024.

Keywords

AuNPs; Diagnosis; ELISA; Flow strip (ICLFS); Immunochromatography lateral; Schistosoma haematobium; Animals; Antibodies, Helminth; Antigens, Helminth; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Gold; Humans; Metal Nanoparticles; Reagent Strips; Schistosomiasis haematobia; Sensitivity and Specificity; helminth antibody; metal nanoparticle; parasite antigen; affinity chromatography; animal; blood; chemistry; enzyme linked immunosorbent assay; human; immunology; procedures; test strip

Citation Information

Scopus Citations: 3

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