Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelrahman S.E.S.A.H.; El Hawary S.; Mohsen E.; El Raey M.A.; Selim H.M.R.M.; Hamdan A.M.E.; Ghareeb M.A.; Hamed A.A.

Journal: Frontiers in Microbiology

Publisher: Frontiers Media SA

Publication Date: 13 May 2024

Volume / Issue: Volume 15

Article No.: 1366614

ISSN: 1664302X

DOI: 10.3389/fmicb.2024.1366614

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abdelrahman S.E.S.A.H., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza, Egypt; El Hawary S., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza, Egypt; Mohsen E., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza, Egypt; El Raey M.A., Department of Phytochemistry and Plant Systematics, Pharmaceutical Division, National Research Centre, Cairo, Egypt; Selim H.M.R.M., Department of Pharmaceutical Sciences, Faculty of Pharmacy, Almaarefa University, Riyadh, Saudi Arabia, Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt; Hamdan A.M.E., Department of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; Hamed A.A., Microbial Chemistry Department, National Research Centre, Giza, Egypt

Abstract

Introduction: In recent years, the world’s attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications. Methods: The purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). Results and Discussion: The HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles’ surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4’-sulfate showed promise as an anti-cancer metabolite. Copyright © 2024 Abdelrahman, El Hawary, Mohsen, El Raey, Selim, Hamdan, Ghareeb and Hamed.

Keywords

anticancer; antimicrobial; Aspergillus sp. SA17; DNA gyrase; docking; phosphoinositide 3-kinase gamma; UPLC-QTOF-MS/MS; ZnONPs; alkaloid; ampicillin; anthraquinone; coumarin derivative; flavonoid; fungal extract; iridoid; lignan; nanomaterial; phenol derivative; streptomycin; xanthone derivative; zinc oxide nanoparticle; antibacterial activity; antibiotic resistance; antineoplastic activity; Article; Aspergillus; bacterium contamination; biosynthesis; cell viability; computer model; cytotoxicity; electron microscopy; endophytic fungus; Fourier transform infrared spectroscopy; fungal strain; fungus isolation; genetic identification; high performance liquid chromatography; human; human cell; mass spectrometry; molecular docking; molecular dynamics; MTT assay; nanofabrication; scanning electron microscopy; transmission electron microscopy; ultra performance liquid chromatography; X ray diffraction; zeta potential; zone of inhibition

Citation Information

Scopus Citations: 18

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