Assessment of antimicrobial activity and mycelium insights of Terfezia claveryi truffle harvested from Tindouf desert

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mohamed-Benkada M.; Achira D.Y.; Ghareeb M.A.

Journal: Journal of Applied Pharmaceutical Science

Publisher: Open Science Publishers LLP Inc.

Publication Date: 1 February 2024

Volume / Issue: Volume 14 / Issue 2

Pages: 161–173

ISSN: 22313354

DOI: 10.7324/JAPS.2024.169988

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Mohamed-Benkada M., Department of Living and Environment, Faculty of Nature and Life Sciences, University of Sciences and Technology of Oran-Mohamed Boudiaf USTO-MB, Oran, Algeria; Achira D.Y., Laboratory of Aquaculture and Bioremediation “AquaBior”, Department of Biotechnology, University Oran1 Ahmed Ben Bella, Oran, Algeria; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

This work focuses on the bioactivity characterization of the edible desert truffle Terfezia claveryi harvested from the Tindouf area in the South-West of Algeria. We investigated the morphological and germination features of T. claveryi spores under light and scanning electron microscopy. Afterward, we isolated the mycelium from a unique spore, which was then used in seedling-optimized in vitro biomass liquid culture. After that, we focused on the antibacterial and antifungal properties of this mushroom (fruit body flesh and mycelium) evaluated respectively by disc Agar diffusion method and Denis and Webster methods I and II. Furthermore, we tested antagonism against plant-pathogenic fungal species using three confrontation methods on a solid medium. Finally, we developed undescribed protocols for extracting, purifying, and describing bioactive compounds. Results showed that developing T. claveryi spore germination, mycelium isolation, and axenic liquid subcultures is very slow. The flesh extract is only antibacterial, while the one from the culture medium is antifungal and antibacterial. The most bioactive extract on bacteria, yeast, and plant-pathogenic fungal strains tested is the one from the mycelium of T. claveryi. The latter strain showed good antagonistic power against some plant-pathogenic fungal tested strains, probably owing to antibiotic component biosynthesis with antifungal ability. © 2024, Mustapha Mohamed-Benkada et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License(https://creativecommons.org/licenses/by/4.0/). All rights reserved.

Keywords

antibacterial; antifungal; chemical characterization; desert; monoclonal mycelium; Tindouf; truffle; plant extract; terfezia claveryi extract; unclassified drug; agar diffusion; analytic method; antibacterial activity; antifungal activity; antimicrobial activity; Article; Aschochyta rabiei; biological activity; biomass; Candida albicans; controlled study; disk diffusion; Escherichia coli; fungal strain; Fusarium oxysporum; Fusarium oxysporum f. sp. lycopersici; germination; high performance liquid chromatography; Klebsiella; mass fragmentography; microfiltration; minimum inhibitory concentration; morphology; mushroom; mycelial growth; mycelium; nonhuman; Phytophtora; Pseudomonas aeruginosa; Pseudomonas fluorescens; Salmonella enterica serovar Typhi; scanning electron microscopy; Sclerotinia sclerotiorum; Shigella; spore germination; spring; Staphylococcus aureus; Streptococcus; Terfezia claveryi truffle; thin layer chromatography; zone of inhibition

Citation Information

Scopus Citations: 4

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