Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hamed A.A.; Ghareeb M.A.; Kelany A.K.; Abdelraof M.; Kabary H.A.; Soliman N.R.; Elawady M.E.

Journal: BMC Biotechnology

Publisher: BioMed Central Ltd

Publication Date: 17 January 2024

Volume / Issue: Volume 24 / Issue 1

Article No.: 3

ISSN: 14726750

DOI: 10.1186/s12896-024-00830-z

Scopus: View on Scopus

PubMed: 38233817

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Hamed A.A., Microbial Chemistry Department, National Research Centre, Dokki, Cairo, 12622, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, (P.O. 30), Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Kelany A.K., Department of Genomic Medicine, Cairo University, Giza, Egypt; Abdelraof M., Microbial Chemistry Department, National Research Centre, Dokki, Cairo, 12622, Egypt; Kabary H.A., Department Agricultural Microbiology, National Research Center, 33 El Buhouth St., Dokki, Giza, 12622, Egypt; Soliman N.R., Dairy Science Department, National Research Center, Cairo, Dokki, Egypt; Elawady M.E., Microbial Biotechnology Department, Biotechnology Research Institute National Research Centre, Cairo, Egypt

Abstract

The growing spread of infectious diseases has become a potential global health threat to human beings. According to WHO reports, in this study, we investigated the impact of co-cultivating the isolated endophytic fungus Aspergillus sp. CO2 and Bacillus sp. COBZ21 as a method to stimulate the production of natural bioactive substances. (GC/MS)-based metabolomics profiling of two sponge-associated microbes, Aspergillus sp. CO2 and Bacillus sp. COBZ21, revealed that the co-culture of these two isolates induced the accumulation of metabolites that were not traced in their axenic cultures. By detection of different activities of extracts of Bacillus sp. COBZ21 and Aspergillus sp. CO2 and coculture between Bacillus sp. COBZ21 and Aspergillus sp. CO2. It was noted that the coculture strategy was the reason for a notable increase in some different activities, such as the antimicrobial activity, which showed potent activity against Escherichia coli ATCC 25,922, Staphylococcus aureus NRRLB-767, and Candida albicans ATCC 10,231. The antibiofilm activity showed significant biofilm inhibitory activity toward Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 10,145, and Staph aureus NRRLB-767, with activity up to 53.66, 71.17, and 47.89%, while it showed low activity against E. coli ATCC 25,922, while the antioxidant activity based on the DPPH assay showed maximum activity (75.25%). GC-MS investigations revealed the presence of variable chemical constituents belonging to different chemical categories, which reflected their chemical diversity. The main components are (+-) cis-Deethylburnamine (2.66%), Bis(3,6,9,12-tetraoxapentaethylene) crowno-N,N,N’,N’-tetra methylpphanediamine (2.48%), and 11-phenyl-2,4,6,8-tetra(2-thienyl)-11-aza-5,13-dithiaeteracyclo[7.3.0.1(2,8)0.0(3,7)] trideca-3,6-diene-10,12,13-trione (3.13%), respectively, for Bacillus sp. axenic culture, Aspergillus sp. CO2, Aspergillus sp. CO2, and Bacillus sp. COBZ21 coculture. By studying the ADME-related physicochemical properties of coculture extract, the compound showed log P o/w values above 5 (8.82). The solubility of the substance was moderate. In order to provide a comprehensive definition of medicinal chemistry and leadlikness, it is important to note that the latter did not meet the criteria outlined in the rule of three (RO3). The toxicity prediction of the coculture extract was performed using the ProTox II web server, which showed that the selected compound has no pronounced toxicity. © 2024, The Author(s).

Keywords

Antibiofilm; Antimicrobial; Antioxidant; Bacteria; Coculture; Fungi; Anti-Bacterial Agents; Anti-Infective Agents; Antioxidants; Aspergillus; Bacillus; Carbon Dioxide; Escherichia coli; Humans; Microbial Sensitivity Tests; Plant Extracts; Staphylococcus aureus; Bacteriology; Cultivation; Ethanol; Health risks; Metabolites; 11 phenyl 2,4,6,8 tetra(2 thienyl) 11 aza 5,13 dithiaeteracyclo[7.3.0.1(2,8)0.0(3,7)] trideca 3,6 diene 10,12,13 trione; bis(3,6,9,12 tetraoxapentaethylene) crowno n,n,n,n tetra methylpphanediamine; deethylburnamine; fungal extract; unclassified drug; antiinfective agent; plant extract; Antibiofilms; Aspergillus sp; Axenic culture; Bacillus sp; Co-cultivation; Co-cultures; Metabolite production; Red sea; Sea sponge; absorption; antibiofilm activity; antimicrobial activity; antioxidant activity; Article; Bacillus subtilis; bacterium isolate; biofilm inhibitory concentration; Candida albicans; controlled study; DPPH radical scavenging assay; drug distribution; excretion; fungal detection; fungus isolation; mass fragmentography; medicinal chemistry; metabolism; metabolite; metabolomics; nonhuman; physical chemistry; prediction; Pseudomonas aeruginosa; solubility; chemistry; human; microbial sensitivity test

Citation Information

Scopus Citations: 32

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