Chemical constituents of ostracod Heterocypris salina extract, anticancer and antimicrobial activity: in silico supported in vitro study

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hegab M.H.; Abdelhameed M.S.; Shawer E.E.; Negm El-Dein A.; Sabour R.; Ghareeb M.A.

Journal: Egyptian Pharmaceutical Journal

Publisher: Wolters Kluwer Medknow Publications

Publication Date: 20 November 2023

Volume / Issue: Volume 23 / Issue 1

Pages: 85–93

ISSN: 16874315

DOI: 10.4103/epj.epj_107_23

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Hegab M.H., National Institute of Oceanography and Fisheries, Freshwater and Lakes Division, NIOF, Cairo, 4262110, Egypt; Abdelhameed M.S., National Institute of Oceanography and Fisheries, Freshwater and Lakes Division, NIOF, Cairo, 4262110, Egypt; Shawer E.E., National Institute of Oceanography and Fisheries, Freshwater and Lakes Division, NIOF, Cairo, 4262110, Egypt; Negm El-Dein A., Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, 12622, Egypt; Sabour R., Department of Medicinal Pharmaceutical Chemistry and Drug Design, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt

Abstract

Background The majority of bioactive compounds derived from the sea have been extracted from aquatic invertebrates. While Ostracods are a diverse class of crustaceans that inhabit marine, brackish, and freshwaters, there are no available studies testing Ostracoda in the medical field. Objective The aim of this work was to produce H. salina on a large scale to evaluate its crude extract for anticancer and antimicrobial activities. Materials and methods Ostracod, Heterocypris salina was collected from its habitat in the eastern side of Lake Manzalla, Egypt, and the species was cultured in Soaba System of the National Institute of Oceanography and Fisheries (NIOF), Al-Khairia City, Egypt). Crude extract of Heterocypris salina was prepared and the extracr was tested as an anticancer against different human cancer cell lines. Also, the extract was investigated as antimicrobial against different Gram-positive and Gram-negative bacteria. In addition, chemical composition of the extract by GC-MS analysis and molecular docking were also studies. Results and conclusion Ostracod Heterocypris salina was cultured, at day 50 of the culturing, about 500 L of the culturing pond was harvested using plankton to collect the biomass of H. salina. A methanol extract of H. salina was prepared and showed a moderate to good anticancer effect against liver (HepG2), lung (A549), colon (HCT), and breast (MCF7) cancer cell lines with IC50 values in the range of 12.8–23.2 μg/ml), being safe for the healthy cells of the same organs. Also, the H. salina extract showed activity against three tested Gram-negative (Escherichia coli, Klebsiella pneumonia, and Salmonella typhi) and two Gram-positive (Sarcina lutea and Bacillus cereus) bacteria. GC-MS analysis of H. salina extract revealed the presence of 11 compounds including ethyl iso-allocholate (23.1%), 9(Z)-octadecenamide, (Z)-(20.6%), oleic acid (12.3%), palmitic acid (11.65%), and α-amyrin (10.73%). Moreover, a molecular docking study was performed in order to determine the possible binding interactions of the test compounds with the essential amino acids in the binding site of thymidylate kinase (TMK). © 2024 Egyptian Pharmaceutical Journal.

Keywords

anticancer; antimicrobial; bioactive compounds; docking; Heterocypris salina; ostracoda; thymidylate kinase; 2 piperidinone; 4 methylvaleric acid; alpha amyrin; amikacin; amoxicillin; animal extract; antibiotic agent; antineoplastic agent; benzenepropanoic acid 1 methylethyl ester; ciprofloxacin; cyclopentaneundecanoic acid methyl ester; cytotoxic agent; essential amino acid; ethyl iso allocholate; methanol; octadecenamide; oleic acid; oleic acid amide; palmitic acid; phosphotransferase; phytol; staurosporine; tetramethyl 2 hexadecen; unclassified drug; A-549 cell line; antibacterial activity; antineoplastic activity; antiproliferative activity; Article; Bacillus cereus; biomass; cancer cell line; chemical composition; computer model; controlled study; drug binding site; drug cytotoxicity; drug isolation; drug protein binding; enzyme active site; Escherichia coli; Hep-G2 cell line; human; human cell; IC50; in vitro study; Klebsiella pneumoniae; mass fragmentography; MCF-7 cell line; Micrococcus luteus; molecular docking; ostracod; plankton; Salmonella enterica serovar Typhi

Citation Information

Scopus Citations: 1

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