Photobiological modulation of hepatoma cell lines and hepatitis B subviral particles secretion in response to 650 nm low level laser treatment

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Al-Toukhy G.M.; Suef R.A.; Hassan S.; Farag M.M.S.; El-Tayeb T.A.; Mansour M.T.M.

Journal: Journal of the Egyptian National Cancer Institute

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 23 October 2023

Volume / Issue: Volume 35 / Issue 1

Article No.: 33

ISSN: 11100362

DOI: 10.1186/s43046-023-00190-3

Scopus: View on Scopus

PubMed: 37870653

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Al-Toukhy G.M., Department of Virology and Immunology, Children’s Cancer Hospital, Cairo, 57357, Egypt; Suef R.A., Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt; Hassan S., Pathology and Electron Microscopy, Theodor Bilharz Research Institute, Giza, Egypt; Farag M.M.S., Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt, Biomedical Research Department, Armed Forces College of Medicine, Cairo, Egypt; El-Tayeb T.A., National Institute of Laser Enhanced Science (NILES), Cairo University, Cairo, Egypt; Mansour M.T.M., Department of Virology and Immunology, National Cancer Institute, Cairo University, Cairo, Egypt, Children Cancer Hospital, Cairo, 57357, Egypt

Abstract

Background: Chronic hepatitis B virus (HBV) infection is a serious global health concern, with an increased incidence and risk of developing cirrhosis and hepatocellular carcinoma (HCC). Patients chronically infected with HBV are likely to experience chronic oxidative stress, leading to mitochondrial dysfunction. Photobiomodulation is induced by the absorption of low-level laser therapy (LLLT) with a red or infrared laser by cytochrome C oxidase enzyme, resulting in mitochondrial photoactivation. Although it is widely used in clinical practice, the use of LLL as adjuvant therapy for persistent HBV infection is uncommon. This study aimed to investigate the effect of LLLT dosage from 2 J/cm2 to 10 J/cm2 of red diode laser (650 nm) on both hepatoma cell lines (HepG2.2.15 [integrated HBV genome stable cell model] and non-integrated HepG2), with a subsequent impact on HBVsvp production. Methods: The present study evaluated the effects of different fluences of low-level laser therapy (LLLT) irradiation on various aspects of hepatoma cell behavior, including morphology, viability, ultrastructure, and its impact on HBVsvp synthesis. Results: In response to LLLT irradiation, we observed a considerable reduction in viability, proliferation, and HBVsvp production in both hepatoma cell lines HepG2.2.15 and HepG2. Ultrastructural modification of mitochondria and nuclear membranes: This effect was dose, cell type, and time-dependent. Conclusions: The use of LLLT may be a promising therapy for HCC and HBV patients by reducing cell proliferation, HBVsvp production, and altering mitochondrial and nuclear structure involved in cellular death inducers. Further research is required to explore its clinical application. © 2023, Egyptian National Cancer Institute.

Keywords

HBVsvps production; Hepatitis B infection; HepG2.2.15 cell line; Human hepatoma HepG2; Low-level laser therapy; Photobiomodulation; Carcinoma, Hepatocellular; Cell Line; Hepatitis B; Hepatitis B virus; Hepatitis B, Chronic; Humans; Liver Neoplasms; Article; cell function; cell nucleus membrane; cell proliferation; cell ultrastructure; cell viability; cell viability percentage; clinical practice; controlled study; Hep-G2/2.2.15 cell line; hepatocellular carcinoma cell line; human; human cell; in vitro study; irradiation; low level laser therapy; mitochondrial membrane; mitochondrion; morphology; morphometry; nonhuman; supernatant; surface area; transmission electron microscopy; virus genome; virus particle; chronic hepatitis B; genetics; liver cell carcinoma; liver tumor; pathology

Citation Information

Scopus Citations: 1

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