HPLC fingerprinting/GC-MS analysis, and efficacy of Hypericum perforatum against cisplatin-induced hepato-renal toxicity in mice with insights into the TXNIP/NLRP3 pathway

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Salem M.B.; Morsi E.A.; El-Wakil E.A.; El-Lakkany N.M.; Abou-shousha T.; Abdel-Hady H.

Journal: Journal of Applied Pharmaceutical Science

Publisher: Open Science Publishers LLP Inc.

Publication Date: 1 September 2023

Volume / Issue: Volume 13 / Issue 9

Pages: 37–47

ISSN: 22313354

DOI: 10.7324/JAPS.2023.6683

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Salem M.B., Pharmacology Department, Theodor Bilharz Research Institute, Giza, Egypt; Morsi E.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; El-Wakil E.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; El-Lakkany N.M., Pharmacology Department, Theodor Bilharz Research Institute, Giza, Egypt; Abou-shousha T., Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Abdel-Hady H., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Hypericum perforatum (HP) has antioxidant and anti-inflammatory characteristics. Nevertheless, its anti-inflammatory effects against hepato-renal injury have not been investigated. Therefore, this survey is designed to evaluate the prophylactic effect of HP extract against cisplatin- (CDDP-) induced hepato-renal toxicity in mice. Also, the active ingredients of HP were recognized. Hepato-renal toxicity was prompted by a single dose of CDDP (13 mg/kg, intraperitoneally). HP was tested at 50, 100, and 200 mg/kg/day for 10 days. Liver and kidney enzymes, oxidative stress, and inflammatory markers were examined, and histopathological examinations were performed. Total phenolic and flavonoid contents, besides 2,2-diphenyl-l-picrylhydrazyl radical scavenging activity, were assessed by identifying HP chemical compounds through high-performance liquid chromatography (HPLC) fingerprinting and gas chromatography-mass spectrometry. HP at 200 mg/kg improved hepatic and renal biochemical markers, reduced oxidative stress, and restored normal liver and kidney histology. Moreover, HP reduced inflammation by inhibiting TXNIP and NLRP3 tissue contents and cleaved caspase-1, interleukin-1β, and nuclear factor kappa-β expressions. The HPLC fingerprinting revealed high concentrations of ellagic acids, cinnamic acids, quercetin, and hesperidin, which may be responsible for alleviating hepato-renal damage. HP is an effective therapeutic agent against CDDP-induced hepato-renal toxicity and could be a realistic adjuvant therapy with CDDP for cancer treatment. © 2023 Maha Badr Salem et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).

Keywords

cisplatin; Hypericum perforatum; inflammasome pathway; oxidative stress; phenolic compounds; alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; biological marker; cinnamic acid; cryopyrin; ellagic acid; flavonoid; hesperidin; Hypericum perforatum extract; immunoglobulin enhancer binding protein; interleukin 1beta; interleukin 1beta converting enzyme; malonaldehyde; phenol derivative; phytochemical; quercetin; thiopental; thioredoxin interacting protein; animal experiment; animal tissue; antioxidant activity; Article; blood analysis; body weight; cancer therapy; DPPH radical scavenging assay; enzyme linked immunosorbent assay; experimental design; high performance liquid chromatography; histology; histopathology; inflammation; kidney injury; liver injury; liver toxicity; male; mass fragmentography; mouse; nephrotoxicity; nonhuman; real time polymerase chain reaction; relative kidney weight; relative liver weight; relative organ weight; retention time; RNA extraction; single drug dose; Swiss Webster mouse

Citation Information

Scopus Citations: 6

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