Cryptosporidium parvum oocytic antigen induces dendrtic cell maturation that suppresses Th2 cytokines when co-cultured with CD4+ cells

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Connick K.; Lalor R.; Murphy A.; Oneill S.; Zalat R.; El Shanawany E.

Journal: Iraqi Journal of Veterinary Sciences

Publisher: University of Mosul - College of Veterinary Medicine

Publication Date: 1 April 2023

Volume / Issue: Volume 37 / Issue 2

Pages: 515–523

ISSN: 16073894

DOI: 10.33899/ijvs.2022.133847.2313

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Connick K., School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; Lalor R., School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; Murphy A., School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; Oneill S., School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; Zalat R., Department of Parasitology, Theodor Bilharz Research Institute, Egypt; El Shanawany E., Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Giza, Egypt

Abstract

Cryptosporidium parvum is an opportunistic intracellular parasite that causes disease in animal populations such as calves and goats. It is also a significant zoonotic disease globally, causing mild to severe human diarrhea. In immunocompromised animals, calves and lambs, and immunocompromised humans such as AIDS patients, an infection can be life-threatening as no effective treatments are currently available to control infection. The effects of Cryptosporidium parvum antigen (CPA) on dendritic cells (DCs) were investigated. This study examined cytokine secretion and cell surface marker expression on DCs exposed to CPA. Cytokine production in CD4+ cells co-cultured with CPA primed DCs in the presence of anti-CD3 was also measured. CPA induced a significant increase in the production of interleukin (IL)-12p40, IL-10, IL-6, and TNF-α by DCs and enhanced the expression of the cell surface markers TLR4, CD80, CD86, and MHC11. CPA primed DC co-cultured in the presence of anti-CD3 with CD4+ T-cells inhibited the secretion of Th2-associated cytokines, notably IL-5 and IL-13, with no effects on the secretions of interferon (IFN)-γ, IL-2, IL-17, and IL-10. These findings support studies in the literature that CPA can induce the full maturation of DCs that subsequently initiate Th1 immune responses critical to the resolution of C. parvum infection. © Authors, 2023, College of Veterinary Medicine, University of Mosul.

Keywords

Cell surface marker; Cryptosporidium parvum; Dendritic cells; antigen; B7 antigen; CD86 antigen; Cryptosporidium parvum antigen; cytokine; endotoxin; gamma interferon; interleukin 10; interleukin 12p40; interleukin 13; interleukin 17; interleukin 2; interleukin 5; interleukin 6; toll like receptor 4; tumor necrosis factor; unclassified drug; animal cell; animal experiment; Article; bone marrow derived dendritic cell; CD4+ T lymphocyte; cell counting; cell isolation; cell maturation; cell suspension; cell viability; coculture; controlled study; cytokine production; cytokine release; cytotoxicity; dendritic cell; enzyme linked immunosorbent assay; female; flow cytometry; infant; mouse; MTS assay; nonhuman; Th1 Th2 balance

Citation Information

Scopus Citations: 12

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