Diagnosis of Toxoplasmosis Using Surface Antigen Grade 1 Detection by ELISA, Nano-Gold ELISA, and PCR in Pregnant Women

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Aly N.S.M.; Kim H.-S.; Marei Y.M.; Elhamshary A.S.; Bayoumi I.R.; Omar R.E.; Mohammed D.A.; Miyoshi S.-I.; Rashed G.A.

Journal: International Journal of Nanomedicine

Publisher: Dove Medical Press Ltd

Publication Date: March 2023

Volume / Issue: Volume 18

Pages: 1335–1345

ISSN: 11769114

DOI: 10.2147/IJN.S401876

Scopus: View on Scopus

PubMed: 36974071

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Aly N.S.M., Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt, Division of International Infectious Diseases Control, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan; Kim H.-S., Division of International Infectious Diseases Control, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan; Marei Y.M., Medical Biochemistry Department, Faculty of Medicine, Benha University, Benha, 13511, Egypt; Elhamshary A.S., Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt; Bayoumi I.R., Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; Omar R.E., Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt; Mohammed D.A., Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt; Miyoshi S.-I., Department of Sanitary Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan; Rashed G.A., Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt

Abstract

Introduction: The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases. Aim and Methods: The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics. Results: PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and 83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no statistical difference between PCR and nanogold ELISA results. Discussion: Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the surface area of nano-gold particles. Conclusion: Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies. © 2023 Aly et al.

Keywords

ELISA; immunodiagnosis; nanogold; PCR; SAG 1; toxoplasmosis; Antibodies, Protozoan; Enzyme-Linked Immunosorbent Assay; Female; Humans; Polymerase Chain Reaction; Pregnancy; Pregnant Women; Toxoplasma; membrane antigen; parasite antigen; protozoon antibody; animal experiment; animal model; Article; controlled study; diagnostic accuracy; diagnostic value; enzyme linked immunosorbent assay; human; major clinical study; male; mouse; nonhuman; outpatient department; pregnant woman; prenatal care; sensitivity and specificity; procedures

Citation Information

Scopus Citations: 21

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