Promoting Efficacy of Human Platelet Lysate on Proliferation of Mammalian Cell Lines Used for Vaccine Production

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Aly N.I.; Fahmy H.M.; Atta O.; Elzallat M.; Abass A.M.; Abu-Taleb H.

Journal: Journal of Advanced Veterinary Research

Publisher: Assiut University, Faculty of Veterinary Medicine, Department of Animal Medicine

Publication Date: 2023

Volume / Issue: Volume 13 / Issue 6

Pages: 1157–1160

ISSN: 20906269

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Aly N.I., Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt; Fahmy H.M., Laboratory and Transfusion Medicine, Ain Shams Medical School, Cairo, Egypt; Atta O., Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt; Elzallat M., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Abass A.M., Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt; Abu-Taleb H., Environmental Research Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

The current study was conducted to determine to what extent human platelet lysate (HPL) can be used to enhance the growth of three cell lines used for propagation of Bovine Ephemeral Fever Virus (BEFV) and Rabies virus (RV) for vaccine production. HPL; Fetal Calf Serum (FCS) and Newborn Calf Serum (NBCS) were used as supplements to the used cell culture medium (Minimum Essential Medium “MEM”) for passaging of three cell lines including baby hamster kidney (BHK-21); African green monkey kidney (VERO) and Madin Derby canine kidney (MDCK). Through three successive cell culture passages; for each cell line; it was found that HPL supported growth and propagation of BHK-21, VERO and MDCK cell lines without altering their morphologic and functional characteristics. It has been accepted as a suitable alternative to FBS and NBCS, and it can be used in vaccinal production. © 2011-2023 Journal of Advanced Veterinary Research. All rights reserved.

Keywords

Cell culture; Growth factor; Human Platelet Lysate (HPL)

Citation Information

Scopus Citations: 2

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