Ameliorative Effects of Thunbergia erecta L. Leaves Against the Initiation of Hepatocarcinogenesis Induced by Diethylnitrosamine in the Rat Model

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Farag F.S.A.-A.; Anwar H.M.; Aboushousha T.; Mohammed H.S.; Ismail L.D.M.

Journal: Applied Biochemistry and Biotechnology

Publisher: Springer

Publication Date: 28 January 2023

Volume / Issue: Volume 195 / Issue 10

Pages: 5881–5902

ISSN: 2732289

DOI: 10.1007/s12010-022-04292-x

Scopus: View on Scopus

PubMed: 36708488

Document Type: Article

Access: All Open Access; Green Open Access; Hybrid Gold Open Access

Authors and Affiliations

Farag F.S.A.-A., Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, 11651, Egypt; Anwar H.M., Department of Biochemistry, National Organization for Drug Control & Research, Cairo, Egypt; Aboushousha T., Department of Pathology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, P.O. 30 Imbaba, Giza, 12411, Egypt; Mohammed H.S., Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, 11651, Egypt; Ismail L.D.M., Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11651, Egypt

Abstract

Thunbergia erecta L. contains cytotoxic and liver-protective compounds. Thunbergia erecta L. leaves were macerated in 70% aqueous ethanol, then fractionated with ethyl acetate (9.3 g) and butanol (12.7 g), and attenuated Den-induced liver cancer in a Wistar rat experimental model. Ethyl acetate and butanol fractions were chromatographed using column chromatography and solid-phase extraction (SPE); Vicenin-II (1), kaempferol (2), biochanin A, sissotrin 7-O-β-glucopyranoside (3), gentianose (4), acacetin 7-O-β-glucopyranoside (5), apigenin 7-O-β-glucopyranoside (6), and rosmarinic acid (7) were extracted, and their structures were determined using NMR spectroscopy and ESI-mass spectrometry. Sixty rats were divided into six groups (ten each): control group, Den group, doxorubicin/Den-treated group, butanol fraction/Den-treated group, and isolated acacetin 7-O-β-glucopyranoside/Den-treated group. The liver enzymes and proinflammatory biomarkers were used to estimate the liver function. In addition, liver tissues were collected for analysis of oxidative stress markers, gene expression, and histopathology. There is a significant increase in the levels of liver enzymes, AFP, and TNF-ἁ. This was conveyed by a significant increase of IL-1 and caspase-3, elevation of MDA and reduction of GSH, and suppression of Bcl2 and elevation of Bax expression. All parameters in butanol, ethyl acetate fractions, and isolated acacetin 7-O-β-glucopyranoside (major constituents) of T. erecta L. were significantly improved to values close to those of the control group. © 2023, The Author(s).

Keywords

Acacetin; Apoptosis; Caspase; Diethylnitrosamine; Phenolic; Thunbergia; Animals; Butanols; Carcinogenesis; Liver; Plant Leaves; Rats; Rats, Wistar; Column chromatography; Diseases; Drug products; Enzymes; Flavonoids; Gene expression; Mass spectrometry; Nuclear magnetic resonance spectroscopy; Phase separation; acacetin 7 o beta glucopyranoside; acetic acid ethyl ester; alanine aminotransferase; alcohol; alpha fetoprotein; antifibrotic agent; antiinflammatory agent; antineoplastic agent; antioxidant; apigenin 7 o beta glucopyranoside; apoptosis inhibitor; aspartate aminotransferase; biochanin A; butanol; caspase 3; doxorubicin; flavonoid; gentianose; glutathione; interleukin 1beta; kaempferol; malonaldehyde; plant extract; plant medicinal product; protein Bax; protein bcl 2; rosmarinic acid; sissotrin 7 o beta glucopyranoside; Thunbergia erecta extract; tumor necrosis factor; unclassified drug; vicenin II; Caspases; Control groups; Ethyl-acetate; Glucopyranoside; Hepatocarcinogenesis; Liver enzymes; Phenolics; Thunbergium; Acanthaceae; adult; alanine aminotransferase blood level; alpha fetoprotein blood level; animal experiment; animal model; animal tissue; antiapoptotic activity; antifibrotic activity; antiinflammatory activity; antineoplastic activity; antioxidant activity; aqueous solution; Article; aspartate aminotransferase blood level; carbon nuclear magnetic resonance; controlled study; diethylnitrosamine-induced hepatocarcinogenesis; drug efficacy; drug mechanism; electrospray mass spectrometry; enzyme activity; ethnopharmacology; gene expression level; histopathology; inflammation; liver cancer; liver fibrosis; liver function; liver tissue; male; nonhuman; oxidative stress; phytochemistry; plant leaf; proton nuclear magnetic resonance; rat; solid phase extraction; structure activity relation; Thunbergia erecta; Wistar rat; animal; chemistry; metabolism; Cell death

Citation Information

Scopus Citations: 3

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