Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Agour M.A.; Hamed A.A.; Ghareeb M.A.; Abdel-Hamid E.A.A.; Ibrahim M.K.

Journal: Archives of Microbiology

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 1 August 2022

Volume / Issue: Volume 204 / Issue 8

Article No.: 537

ISSN: 3028933

DOI: 10.1007/s00203-022-03092-5

Scopus: View on Scopus

PubMed: 35913539

Document Type: Article

Access: All Open Access; Green Open Access; Hybrid Gold Open Access

Authors and Affiliations

Agour M.A., Botany Department, Faculty of Science, Zagazig University, Zagazig, Egypt; Hamed A.A., Microbial Chemistry Department, National Research Centre, 33 El-Buhouth Street, Dokki, Giza, 12622, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El Nile, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Abdel-Hamid E.A.A., Central Laboratory for Aquaculture Research, Abbassa, Abu-Hammad, Sharqia, Egypt; Ibrahim M.K., Microbiology Department, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt

Abstract

This study was designed to evaluate the antimicrobial, antioxidant, and cytotoxic potentials of the marine actinomycetes spp. isolated from the Red Sea water, Hurghada, Egypt. Out of 80 actinomycetes isolates, one isolate AW6 was selected based on its antioxidant activity (IC50 about 5.24 µg/mL which scavenged 91% of formed DPPH free radicals) and antimicrobial potential against E. coli, S. aureus, B. subtilis, and P. aeruginosa, A. niger, and C. albicans. The strain was identified based on phenotypic and genotypic analysis, and deposited in the GenBank with accession number OK090864.1. Cultivation of the selected strain on rice, chromatographic purification, and structural elucidation led to the isolation of two compounds C1: umbelliferone, and C2: 1-methoxy-3-methyl-8-hydroxy-anthraquinone. The antimicrobial activity of the obtained compounds showed that C1 and C2 have low antibacterial activity toward S. aureus and E. coli with no pronounced activity toward P. aeruginosa, C. albicans, and A. niger. Additionally, the antioxidant activity of C1 and C2 revealed that C2 has a good antioxidant activity, with DPPH scavenging activity reaching (55.25%), followed by C1 (30.20%). Moreover, both compounds displayed anti-Gyr-B enzyme activity with IC50 value of (3.79 ± 0.21 µM) for C1, and (IC50 = 13 ± 0.71 µM) for C2. The ADME-related physicochemical properties of the obtained compound were predicted using SwissADME web tools and the ProToxii webserver was used to estimate in silico toxicity. © 2022, The Author(s).

Keywords

Actinomyces sp. AW6; ADME; Anti-Gyr activity; Antimicrobial; Antioxidant; Secondary metabolites; Actinomyces; Anti-Bacterial Agents; Anti-Infective Agents; Antioxidants; Candida albicans; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus; acetic acid ethyl ester; antiinfective agent; DNA; DNA 16S; DNA topoisomerase (ATP hydrolysing) B; free radical; glycerol; nitrate; nystatin; parathion; RNA 16S; salt water; sephadex; starch; Taq polymerase; umbelliferone; Actinobacteria; antibacterial activity; antimicrobial activity; antioxidant activity; Article; Aspergillus niger; Bacillus subtilis; bacterial strain; bacterium culture; bacterium isolate; computer model; controlled study; Egypt; enzyme activity; GenBank; IC50; methicillin resistant Staphylococcus aureus; nonhuman; phenotype; physical chemistry; Red Sea; rice; chemistry; genetics; metabolism

Citation Information

Scopus Citations: 22

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