In vitro and in vivo impacts of nifedipine and diltiazem on praziquantel chemotherapy in murine Schistosoma mansoni

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Adel Madbouly N.; Emam M.; Ayman M.; Ayman M.; Rabia I.; El Amir A.

Journal: Experimental Parasitology

Publisher: Academic Press Inc.

Publication Date: May 2022

Volume / Issue: Volume 236-237

Article No.: 108256

ISSN: 144894

DOI: 10.1016/j.exppara.2022.108256

Scopus: View on Scopus

PubMed: 35398100

Document Type: Article

Authors and Affiliations

Adel Madbouly N., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Emam M., Faculty of Biotechnology, October University for Modern Science and Arts (MSA), 6th October City, Giza, Egypt; Ayman M., Faculty of Biotechnology, October University for Modern Science and Arts (MSA), 6th October City, Giza, Egypt; Ayman M., Faculty of Biotechnology, October University for Modern Science and Arts (MSA), 6th October City, Giza, Egypt; Rabia I., Department of Parasitology, Theodore Bilharz Research Institute, Giza, Egypt; El Amir A., Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt

Abstract

Aim: This study was planned to evaluate the in vitro and in vivo antischistosomal effects of the widely used antihypertensive drugs, nifedipine (NIF) and diltiazem (DTZ), and their combinations with praziquantel (PZQ) on early and late Schistosoma (S.) mansoni infections 21- and 45- days old stages. Methods: In the In vitro study, Calcium channel blockers (CCBs), NIF and DTZ were added to schistosomula and adult worm cultures in different concentrations 10, 20 and 30 mg/ml. The mortality percentage was calculated 1, 12 and 24 h after incubation. In vivo, NIF and DTZ either alone or combined with PZQ were used to treat male albino mice. The parasitological and total immunoglobulin (Ig) G and IgM anti-soluble egg antigen (SEA) were assessed to demonstrate the disease severity. Results: In the In vitro study, 10 mg/ml NIF induced 100% mortality percentage of both schistosomula and adult worms after 24 h incubation, while DTZ induced similar mortality percentage at 30 mg/ml concentration. In vivo results showed that early or late combination of 30 mg/kg of NIF, but not DTZ, significantly (P <0.05) enhanced the reductive efficacy of PZQ based on the parasitological data. The maximal reduction (P <0.05) of anti-SEA IgM and IgG levels was developed during NIF-PZQ administration 21- (1.12 ± 0.06 and 1.09 ± 0.04, respectively) or 45- (1.00 ± 0.03 and 0.8 ± 0.06, respectively) days post infection (PI), compared to either PZQ or NIF individual treatments. The decreased concentration of anti-SEA antibodies was correlated with the diminished granulomatous diameter and disease severity. Conclusion: Nifedipine improved PZQ chemotherapy targeting either early or late S. mansoni infection in mice compared to the PZQ mono-therapy. Administering NIF can be considered as a promising drug candidate for schistosomiasis chemotherapy. © 2022

Keywords

Diltiazem; Murine; Nifedipine; Praziquantel; S. mansoni; Sm.TRPM<sub>PZQ</sub>; Animals; Anthelmintics; Immunoglobulin G; Immunoglobulin M; Male; Mice; Schistosoma mansoni; Schistosomiasis mansoni; delay tiazem; epilat; transient receptor potential channel M; anthelmintic agent; adult; animal experiment; animal model; animal tissue; antibody detection; Article; calcium transport; controlled study; disease severity; drug efficacy; drug safety; immunoassay; immunoglobulin blood level; in vitro study; in vivo study; mouse; nonhuman; stereomicroscopy; animal

Citation Information

Scopus Citations: 2

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