Phytochemical investigations, antioxidant, cytotoxic, antidiabetic and antibiofilm activities of Kalanchoe laxiflora flowers

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Osman E.E.A.; Mohamed A.S.; Elkhateeb A.; Gobouri A.; Abdel-Aziz M.M.; Abdel-Hameed E.-S.S.

Journal: European Journal of Integrative Medicine

Publisher: Elsevier GmbH

Publication Date: January 2022

Volume / Issue: Volume 49

Article No.: 102085

ISSN: 18763820

DOI: 10.1016/j.eujim.2021.102085

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Osman E.E.A., Department of Medicinal Chemistry, Theodor Bilharz Institute, Kornaish El-Nile, Warrak El-Hadar, 12411, Imbaba (P.O. Box 30), Giza, Egypt; Mohamed A.S., Department of Medicinal Chemistry, Theodor Bilharz Institute, Kornaish El-Nile, Warrak El-Hadar, 12411, Imbaba (P.O. Box 30), Giza, Egypt; Elkhateeb A., Department of Phytochemistry and Plant Systematics, National Research Centre, 33 El Bohouth St., Dokki, Giza, Egypt; Gobouri A., Department of Chemistry, Faculty of Science, Taif University, Saudi Arabia; Abdel-Aziz M.M., Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, 11651, Egypt; Abdel-Hameed E.-S.S., Department of Medicinal Chemistry, Theodor Bilharz Institute, Kornaish El-Nile, Warrak El-Hadar, 12411, Imbaba (P.O. Box 30), Giza, Egypt

Abstract

Introduction: Kalanchoe laxiflora (Family Crassulaceae) contains different bioactive phytochemicals, which possess several biological activities. The present study aimed at assessing the antioxidant, cytotoxic, antidiabetic, and antibiofilm activities of Kalanchoe laxiflora flower extracts, as well as identification of their phytochemicals. Methods: The total phenolic and flavonoid contents of the tested extracts were investigated using Folin-Ciocalteu's and aluminum trichloride methods, while the antioxidant, cytotoxic, antidiabetic and antibiofilm activities were evaluated using different biomedical assays. The phytoconstituents of K. laxiflora flower extracts were profiled using LC-ESI(-ve)-MS analysis. Results: The phytochemical investigations showed that the n-BuOH fraction had the highest total phenolic content (397.25 ± 1.18 mg GAE / g ext.) and flavonoid content (99.71 ± 0.24 mg RE / g ext.). The n-BuOH fraction also possessed the most potent antioxidant activities (DPPH; SC50=18.72 ± 1.81 µg/mL, RPA; 78.26 ± 0.49 mg AAE /g ext.), and TAC; 446.37±0.23 mg AAE /g ext.) and cytotoxic potential in HepG2 cells with IC50= 9.15±0.33 μg/mL. Furthermore, it exhibited the strongest α-amylase and α-glucosidase inhibitory activities (IC50= 54.72±0.96 µg/mL and 89.02±0.99 µg/mL, respectively). Whereas, the 85% methanolic extract exhibited the highest antibiofilm activity against Escherichia coli (MBIC100= 15.63 µg/ml). Additionally, the LC-ESI(-ve)-MS analysis of K. laxiflora extracts led to the characterization of a total of 35 compounds classified as flavonoid glycosides, gallotannins, alkaloids and fatty acids, which were considered to be the major components. Conclusions: K. laxiflora extracts reduce ROS, improve antidiabetic responses and inhibit bacterial growth. The identification of K. laxiflora specific phytoconstituents will provide a starting point to carry out further in vivo studies on these extracts. © 2021 Elsevier GmbH

Keywords

Antibiofilm; Anticancer; Antidiabetic; Antioxidants; DPPH; Kalanchoe laxiflora; alpha glucosidase inhibitor; amylase inhibitor; antidiabetic agent; antioxidant; cytotoxic agent; digalloyl o deoxyhexose; digalloyl o shikimic acid; digalloylhexose; epigallocatechin gallate; eriodictyol 7 o hexose o deoxyhexose; eriodictyol 7 o hexoside; flower extract; gallic acid; gallic acid glucuronide; gallocatechin methyl gallate; kampferol 3 o deoxyhexose pentoside; luteolin; monogalloyl o acetylgluconic acid; monogalloyl o deoxyhexose; myrcetine 3 o hexose malic acid; myrcetine 3 o rutinoside; myricetin 3 o deoxyhexose; n' caffeoyl n'', n''' dicoumaroyl spermidine; naringenin 7 o acetylhexoside; naringenin 7 o rutinoside; nicotiflorin; oxo dihydroxyn octadecenoic acid; patuletine 3 o hexoside; phytochemical; quercetin 3 o beta dextro glucoside; quercetin 3 o glucuronoide; quercetin 3 o hexose protocatcheuic acid; quercetin 3 o hexoside acetyl deoxyhexose; quercetin 3 o pentoside deoxyhexose; quercetin derivative; unclassified drug; antibiofilm activity; antidiabetic activity; antioxidant activity; Article; comparative study; cytotoxicity; DPPH radical scavenging assay; drug determination; drug identification; electrospray mass spectrometry; Escherichia coli; flower; Hep-G2 cell line; IC50; in vitro study; Kalanchoe; liquid chromatography-mass spectrometry; nonhuman; qualitative analysis; quantitative analysis

Citation Information

Scopus Citations: 17

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