Ki-67 pulmonary immunoreactivity in silver nanoparticles toxicity: Size-rate dependent genotoxic impact

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Ali S.A.; Kadry M.O.; Hammam O.; Hassan S.A.; Abdel-Megeed R.M.

Journal: Toxicology Reports

Publisher: Elsevier Inc.

Publication Date: 2022

Volume / Issue: Volume 9

Pages: 1813–1822

ISSN: 22147500

DOI: 10.1016/j.toxrep.2022.09.011

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Ali S.A., Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St., Dokki, Cairo, 12622, Egypt; Kadry M.O., Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St., Dokki, Cairo, 12622, Egypt; Hammam O., Pathology Department, Theodor Bilharz Research Institute, Egypt; Hassan S.A., Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St., Dokki, Cairo, 12622, Egypt; Abdel-Megeed R.M., Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St., Dokki, Cairo, 12622, Egypt

Abstract

Engineered nanoparticles have been recently utilized in numerous domains particularly, silver nanoparticles (AgNPs). Nonetheless, the possible side effects resulting from AgNPs exposure are not fully clarified. The present study was designed to clarify the toxicity of AgNPs on lung tissue. Furthermore, therapeutic impact of Glycosmis pentaphylla (G. pentaphylla) and Casimiroa edulis (C. edulis) leaves extracts in addition to mucilage and protein (the purified compounds from C. edulis) was investigated against AgNPs induced pulmonary toxicity. Male Swiss albino mice were administered AgNPs orally in two different particle sizes (20 nm and 100 nm) for one month and was further treated via G. pentaphylla, C. edulis, mucilage and protein in a dose of 500 mg/ kg for three weeks. Biochemical, molecular, immunohistochemistry, and histopathological investigations were further assessed. An obvious alteration in oxidative stress biomarkers as well as mRNA gene expression of both survivin and matrix metalloproteinase (MMP-9) was recorded in AgNPs intoxicated group. In addition to, exploration of positive nuclei for Ki-67 was also observed upon AgNPs intoxication. Data declared a significant improvement in the assessed parameters upon G. pentaphylla, C. edulis, mucilage and protein treatment. In conclusion; G. pentaphylla and C. edulis extracts could be considered as a promising candidate as therapeutic regimen against pulmonary toxicity induced via AgNPs due to their enrichment with different active constituents. Practical applications: Due to the expansion of AgNPs applications, it is urgent to investigate their toxic impact associated with release of free silver ions. Different particle sizes of AgNPs can induce various alterations in cellular biochemical parameters, mRNA gene expression, histopathological and immunohistopathological examination. Herein, this natural products extracts are used for the first time as promising therapeutic regimen to ameliorate the toxic effect in AgNPs intoxicated lung tissue in mice model as a result of the bioactive metabolites, especially flavonoids and polyphenolic compounds. © 2022 The Authors

Keywords

AgNPs; Casimiroa edulis; Glycosmis pentaphylla; Ki-67; Matrix metalloproteinase; Survivin; anticonvulsive agent; antioxidant; baculoviral IAP repeat containing protein 5; casimiroa edulis extract; flavonoid; gelatinase B; glycosmis pentaphylla extract; Ki 67 antigen; plant extract; polyphenol derivative; silver; silver nanoparticle; unclassified drug; animal experiment; animal model; animal tissue; antioxidant assay; Article; biochemical analysis; controlled study; drug toxicity; gene expression; genotoxicity; histopathology; immunohistochemistry; immunoreactivity; intoxication; lung parenchyma; lung toxicity; male; metabolite; microscopy; mouse; mouse model; mRNA expression level; mucilage; nonhuman; oxidative stress; particle size; plant leaf; protein purification; silver nanoparticles toxicity; statistical analysis; Swiss Webster mouse; transmission electron microscopy; vascular plant

Citation Information

Scopus Citations: 5

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