Synthesis and characterization of amoxicillin-loaded polymeric nanocapsules as a drug delivery system targeting Helicobacter pylori

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelghany A.; El-Desouky M.A.; Shemis M.

Journal: Arab Journal of Gastroenterology

Publisher: Elsevier Ltd

Publication Date: December 2021

Volume / Issue: Volume 22 / Issue 4

Pages: 278–284

ISSN: 16871979

DOI: 10.1016/j.ajg.2021.06.002

Scopus: View on Scopus

PubMed: 34509390

Document Type: Article

Authors and Affiliations

Abdelghany A., Biochemistry & Molecular Biology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; El-Desouky M.A., Chemistry Department, Faculty of Science, Cairo University, Giza, 12613, Egypt; Shemis M., Biochemistry & Molecular Biology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt

Abstract

Background and study aims: Helicobacter pylori (H. pylori) is well known as the main cause of gastritis, gastroduodenal ulcers, gastric mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Approximately 50% of the world's population is infected with H. pylori. In Egypt, a high prevalence of H. pylori infections has been reported in the general population. This study aimed to prepare amoxicillin-loaded poly (ɛ-caprolactone) nanocapsules to increase its gastric stability and therapeutic activity of the molecule against H. pylori. Materials and methods: In this study, we used the water–oil–water double-emulsion technique to prepare spherical-shaped polymeric nanocapsules containing amoxicillin trihydrate as the core substance and biodegradable biocompatible poly (ɛ-caprolactone) as the shell material. Results: The encapsulation efficiency obtained was 97.2% ± 0.8%. The hydrodynamic diameter of the prepared nanocapsules was 287 ± 8 nm with a positive zeta potential. In vitro release studies indicated that the polymeric nanocapsules showed decreased release percentages at pH 1.2, simulating the gastric fluid while relatively increased release at pH 7.0 where the H. pylori reside. The in vitro antibacterial assay showed better efficiency for amoxicillin nanocapsules than for the uncapsulated free amoxicillin, no efficiency was detected for the PCL nanocapsules indicated that the antibacterial due to amoxicillin alone. Cytotoxicity studies demonstrated less cytotoxicity for the polymeric nanocapsules in comparison with amoxicillin. Conclusions: In conclusion, we have demonstrated that biodegradable polymeric nanocapsules are useful drug delivery agents for increasing the gastric stability and therapeutic activity of amoxicillin trihydrate against H. pylori. © 2021 Pan-Arab Association of Gastroenterology

Keywords

Amoxicillin trihydrate; Double emulsion; Helicobacter pylori; Poly (ɛ-caprolactone); Polymer–drug nanocapsules; Amoxicillin; Drug Delivery Systems; Helicobacter Infections; Humans; Nanocapsules; biomaterial; nanocapsule; nanoshell; oil; polycaprolactone; water; animal cell; Article; bacterial growth; bactericidal activity; bacteriostatic activity; biodegradability; cell viability percentage; controlled study; cytotoxicity; drug delivery system; Gram negative bacterium; hydrodynamics; in vitro study; nanoemulsion; nanoencapsulation; nanopharmaceutics; nonhuman; particle size; pH; polymerization; stomach juice; sustained drug release; Vero cell line; zeta potential; zone of inhibition; Helicobacter infection; human

Citation Information

Scopus Citations: 25

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