The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Haque O.; Pendexter C.A.; Wilks B.T.; Hafiz E.O.A.; Markmann J.F.; Uygun K.; Yeh H.; Tessier S.N.

Journal: Scientific Reports

Publisher: Nature Research

Publication Date: 30 November 2021

Volume / Issue: Volume 11 / Issue 1

Article No.: 23128

ISSN: 20452322

DOI: 10.1038/s41598-021-02417-6

Scopus: View on Scopus

PubMed: 34848781

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Haque O., Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 51 Blossom St, Boston, 02114, MA, United States, Shriners Hospitals for Children, Boston, MA, United States, Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA, United States, Department of Surgery, Division of Transplantation, Center for Transplantation Science, Massachusetts General Hospital, 32 Fruit Street, Boston, 02114, MA, United States, Harvard Medical School, Boston, MA, United States; Pendexter C.A., Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 51 Blossom St, Boston, 02114, MA, United States, Shriners Hospitals for Children, Boston, MA, United States; Wilks B.T., Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 51 Blossom St, Boston, 02114, MA, United States, Shriners Hospitals for Children, Boston, MA, United States, Harvard Medical School, Boston, MA, United States; Hafiz E.O.A., Department of Electron Microscopy Research, Theodor Bilharz Research Institute, Giza, Egypt; Markmann J.F., Department of Surgery, Division of Transplantation, Center for Transplantation Science, Massachusetts General Hospital, 32 Fruit Street, Boston, 02114, MA, United States, Harvard Medical School, Boston, MA, United States; Uygun K., Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 51 Blossom St, Boston, 02114, MA, United States, Shriners Hospitals for Children, Boston, MA, United States, Harvard Medical School, Boston, MA, United States; Yeh H., Department of Surgery, Division of Transplantation, Center for Transplantation Science, Massachusetts General Hospital, 32 Fruit Street, Boston, 02114, MA, United States, Harvard Medical School, Boston, MA, United States; Tessier S.N., Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 51 Blossom St, Boston, 02114, MA, United States, Shriners Hospitals for Children, Boston, MA, United States, Harvard Medical School, Boston, MA, United States

Abstract

In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush. © 2021, The Author(s).

Keywords

Adenosine; Allografts; Allopurinol; Animals; Blood Cells; Cold Ischemia; Cold Temperature; Gastroenterology; Glutathione; Insulin; Liver; Liver Transplantation; Organ Preservation; Organ Preservation Solutions; Perfusion; Raffinose; Rats; Rats, Sprague-Dawley; Ringer's Lactate; Tissue Donors; preservation solution; University of Wisconsin-lactobionate solution; adverse event; allograft; animal; blood cell; cold; comparative study; donor; drug effect; pathology; procedures; rat; Sprague Dawley rat

Citation Information

Scopus Citations: 6

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