Expression of Myoglobin in Normal and Cancer Brain Tissues: Correlation With Hypoxia Markers

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Elsherbiny M.E.; Shaaban M.; El-Tohamy R.; Elkholi I.E.; Hammam O.A.; Magdy M.; Allalunis-Turner J.; Emara M.

Journal: Frontiers in Oncology

Publisher: Frontiers Media S.A.

Publication Date: 30 April 2021

Volume / Issue: Volume 11

Article No.: 590771

ISSN: 2234943X

DOI: 10.3389/fonc.2021.590771

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Elsherbiny M.E., Department of Pharmacology and Toxicology, Ahram Canadian University, 6th of October, Egypt; Shaaban M., Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt; El-Tohamy R., Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt; Elkholi I.E., Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt; Hammam O.A., Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt; Magdy M., Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt; Allalunis-Turner J., Department of Oncology, Faculty of Medicine Dentistry, University of Alberta, Edmonton, AB, Canada; Emara M., Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt

Abstract

Background: Myoglobin (MB) is increasingly recognized as a key player in cancer growth and metastasis. Low oxygen tensions, commonly associated with highly aggressive and recurrent cancers, have been shown to regulate its expression in several cancers such as lung, neck, prostate and breast cancer. However, it is not yet known whether it contributes to the growth and spread of brain cancers especially Glioblastoma multiforme (GBM). Methods: Here we investigate the expression of MB, and its correlation with the hypoxia markers carbonic anhydrase IX (CAIX) and lactate dehydrogenase A (LDHA), in human tissue microarrays of multiple organ tumors, brain tumors, and GBM tumors, and their respective cancer-adjacent normal tissues. Correlation between MB protein expression and tumor grade was also assessed. Results: We show that MB protein is expressed in a wide variety of cancers, benign tumors, cancer-adjacent normal tissues, hyperplastic tissue samples and normal brain tissue, and low oxygen tensions modulate MB protein expression in different brain cancers, including GBM. Enhanced nuclear LDHA immune-reactivity in GBM was also observed. Finally, we report for the first time a positive correlation between MB expression and brain tumor grade. Conclusion: Our data suggest that hypoxia regulate MB expression in different brain cancers (including GBM) and that its expression is associated with a more aggressive phenotype as indicated by the positive correlation with the brain tumor grade. Additionally, a role for nuclear LDHA in promoting aggressive tumor phenotype is also suggested based on enhanced nuclear expression which was observed only in GBM. © Copyright © 2021 Elsherbiny, Shaaban, El-Tohamy, Elkholi, Hammam, Magdy, Allalunis-Turner and Emara.

Keywords

carbonic anhydrase IX; glioblastoma multiforme; human tissue microarray; lactate dehydrogenase A; myoglobin; 3467; 3582; 5648; 62470100gf; ab77232; biological marker; buffer; carbonate dehydratase IX; lactate dehydrogenase; monoclonal antibody; peroxidase; s080; s1964; s2023; s3309; antigen retrieval; Article; controlled study; glioblastoma; human; human tissue; hypoxia; immunohistochemistry; protein expression; tissue microarray

Citation Information

Scopus Citations: 9

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