Punica granatum and amygdalin extracts plus cobalamin combined with albendazole reduce larval burden and myositis in experimental trichinosis; [Extratos de punica granatum e amígdalina mais cobalamina combinado com albendazol reduzem a carga larvar e a miosite na triquinose experimental]

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Esmat M.; Abdel-Aal A.A.; Shalaby M.A.; Fahmy M.-E.A.; Badawi M.A.M.; Elmallawany M.A.; Magdy M.; Afife A.A.; Shafi I.R.A.

Journal: Revista Brasileira de Parasitologia Veterinaria

Publisher: Brazilain Coll Veterinary Parasitology

Publication Date: 2021

Volume / Issue: Volume 30 / Issue 4

Article No.: e012021

ISSN: 0103846X

DOI: 10.1590/S1984-29612021084

Scopus: View on Scopus

PubMed: 34730610

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Esmat M., Department of Medical Parasitology, Faculty of Medicine, Misr University for Science and Technology, 6th October city, Egypt; Abdel-Aal A.A., Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt, Department of Postgraduate Studies & Scientific Research, Armed Forces College of Medicine – AFCM, Cairo, Egypt; Shalaby M.A., Medical Parasitology Department, Theodor Bilharz Research Institute – TBRI, Giza, Egypt; Fahmy M.-E.A., Medical Parasitology Department, Theodor Bilharz Research Institute – TBRI, Giza, Egypt; Badawi M.A.M., Department of Pathology, National Research Center, Giza, Egypt; Elmallawany M.A., Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt; Magdy M., Department of Pathology, Theodor Bilharz Research Institute – TBRI, Giza, Egypt; Afife A.A., College of Life Sciences, Faculty of Medicine, Leicester University, Leicester, United Kingdom; Shafi I.R.A., Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Trichinellosis is a zoonosis results from eating raw or semi-cooked meat of infected animals. Medicinal plants have been used lately as alternatives and/or combined therapies to resolve some drawbacks of the current regimens. This work analyzed the effect of albendazole monotherapy on Trichinella spiralis experimental infection (group A), in comparison to P. granatum and amygdalin extracts +cobalamin (group B), plus its combination with albendazole (group C). The study revealed that the extracts alone or combined with albendazole had an inferior effect to albendazole monotherapy regarding number of adult worms (40.83 ±3.82, 18.67 ±1.86 and 16.83 ±2.32, respectively). However, their effect was more obvious in muscle phase combined with albendazole, achieving the lower number of larvae/mL tissue homogenate (22.33 ±3.27 in comparison to 39.67 ±2.58 achieved by albendazole monotherapy). The extracts exerted a significant immunomodulatory effect by reducing the local CD4+ expression in the intestine as well as in muscle phase (1.15 ±0.25 and 3.80 ±0.65 in comparison to 4.97 ±0.37 and 12.20 ±0.87 with albendazole monotherapy, respectively). So, these extracts improved the therapeutic efficacy of albendazole, specifically in muscle phase and counteracted the inflammatory reaction caused by albendazole monotherapy, thus extensively alleviating the resulting myositis. © 2021, Brazilain Coll Veterinary Parasitology. All rights reserved.

Keywords

Albendazole; Amygdalin; Cobalamin; Muscle phase; Punica grantaum; Trichinosis; Animals; Disease Models, Animal; Larva; Myositis; Plant Extracts; Pomegranate; Trichinella spiralis; Trichinellosis; Vitamin B 12; antiinfective agent; CD4 antigen; plant extract; punica grantaum extract; unclassified drug; cyanocobalamin; animal experiment; animal model; animal tissue; Article; controlled study; drug effect; experimental infection; female; histopathology; immunohistochemistry; intestine; intestine parasite; medicinal plant; monotherapy; mouse; nonhuman; protein expression; tissue homogenate; animal; disease model; veterinary medicine

Citation Information

Scopus Citations: 8

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