Bioactive secondary metabolite from endophytic aspergillus tubenginses ash4 isolated from hyoscyamus muticus: antimicrobial, antibiofilm, antioxidant and anticancer activity

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Elkhouly H.I.; Hamed A.A.; El Hosainy A.M.; Ghareeb M.A.; Sidkey N.M.

Journal: Pharmacognosy Journal

Publisher: EManuscript Technologies

Publication Date: 4 March 2020

Volume / Issue: Volume 13 / Issue 2

Pages: 434–442

ISSN: 9753575

DOI: 10.5530/pj.2021.13.55

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Elkhouly H.I., Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt; Hamed A.A., Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, NRC, Dokki-Giza, Egypt; El Hosainy A.M., Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Imbaba, Giza, Egypt; Sidkey N.M., Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt

Abstract

Background: Endophytes are the richest sources of natural compounds, showing biological varieties and pharmacological activities. Objective: This study aims to isolate bioactive secondary metabolites from endophytic Aspergillus tubenginses with study the biological activity of the isolated bioactive compounds. Materials and Methods: Anofinic acid were obtained from Aspergillus tubenginses crude extract using chromatographic techniques and characterized by spectral analysis. Results: Nine endophytic fungi were isolated from Hyoscyamus muticus plant. The most efficient isolate was AF3 identified as Aspergillus tubenginses ASH4 by 18S rRNA gene sequencing. Anofinic acid is an isolated active metabolite biosynthesized by A. tubenginsis was extracted from ethyl acetate with UPAC name of 2,2-dimethyl-2H-1-benzopyran-6-carboxylic acid. It shows a strong antimicrobial activity against human pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans and Bacillus subtilis. Moreover, anofinic acid inhibits biofilm formation and has antioxidant activity, with strong activity against some carcinoma cells such as HCT-116, Hep-G2 and MCF-7. Conclusion: Anofinic acid was purified from the endophytic Aspergillus tubenginses crude extract and showed antimicrobial, antibiofilm, antioxidant, anticancer activities. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Keywords

Aspergillus tubenginses; Bioactive secondary metabolite; Endophytes; Hyoscyamus muticus; 4 hydroxy phthalic acid dimethyl ester; 5 (hydroxymethyl)furan 2 carbaldehyde; amphotericin; ampicillin; anofinic acid; colchatetralene; doxorubicin; ergosterol; fungal extract; gentamicin; RNA 18S; unclassified drug; vancomycin; antibiofilm activity; antineoplastic activity; antioxidant activity; Article; Aspergillus; Bacillus subtilis; Candida albicans; controlled study; endophytic fungus; Escherichia coli; gene sequence; HCT 116 cell line; Hep-G2 cell line; human; human cell; Hyoscyamus; IC50; MCF-7 cell line; nonhuman; Pseudomonas aeruginosa; Staphylococcus aureus; THP-1 cell line

Citation Information

Scopus Citations: 59

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