UPLC-ESI-MS Analysis of Secondary Metabolites from Tamarix Aphylla With Its In-Vitro Anti-Inflammatory and Anti-Microbial Activities
Bibliographic Information
Authors: Hamed M.M.; El-Wakil E.A.; Abdel-Aziz M.S.; Elshahid Z.A.; Abdel-Hady H.
Journal: Egyptian Journal of Chemistry
Publisher: National Information and Documentation Centre
Publication Date: 20 May 2024
Volume / Issue: Volume 67 / Issue 9
Pages: 1–12
ISSN: 4492285
DOI: 10.21608/EJCHEM.2024.271590.9403
Scopus: View on Scopus
Document Type: Article
Authors and Affiliations
Hamed M.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Warrak El-Hadder, Imbaba, Giza, 12411, Egypt; El-Wakil E.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Warrak El-Hadder, Imbaba, Giza, 12411, Egypt; Abdel-Aziz M.S., Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Dokki 12622, Cairo, Egypt; Elshahid Z.A., Chemistry of Natural and Microbial Products, Pharmaceutical Industries and Drug Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt; Abdel-Hady H., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Warrak El-Hadder, Imbaba, Giza, 12411, Egypt
Abstract
Tamarix aphylla is considered one of the known species of the genus Tamarix. It has been shown to has a various pharmacological property. In our investigation, we study the different extracts of Tamarix aphylla [85% methanol (MeOH), petroleum ether (PE), ethyl acetate (EtOAc), butanol (BuOH), and water (Aqueous) extract] as anti-inflammatory and antimicrobial with the identification of the chemical profile of the most effective extracts. The obtained fractions were subjected to the total phenolic content determination using Folin-Ciocalteu method, the total Flavonoid content was achieved using Aluminum chloride, anti-inflammatory activity was measured by Cell Culture (Seeding and Treatment), and antimicrobial activity was premeditated. Qualitative determination of compounds by Ultra performance liquid chromatography (UPLC-ESI-MS). Our results showed that; the EtOAc and BuOH extracts have the highest phenolic content between the examined extracts. EtOAc extract antimicrobial activity ranged from 27 mm against Escherichia coli, to 19 mm against Aspergillus niger. Moreover, BuOH, EtOAc, and Aqueous extracts proved anti-inflammatory efficacy as induced marked inhibition of NO production by (60-70%) as compared to LPS. UPLC-ESI-MS analysis of the butanol extract in the negative-ion Mode gave compounds such as Gallic acid, while in the positive-ion mode of the butanol extract contains metabolites like syringic acid. Analysis of the Ethyl acetate extract in the negative-ion Mode was 2, 3-Dihydroxybenzoic acid and others. Analysis of the ethyl acetate extract in the positive-ion Mode revealed some natural compounds within gallic acid methyl ester. However, these bioactive compounds are responsible for promising inhibition of Nitric oxide (NO) production, antimicrobial and antibacterial activities. © 2024 National Information and Documentation Center (NIDOC)
Keywords
Anti-inflammatory; Antibacterial activity; T. aphylla; Total flavonoid content; Total phenolic content
Citation Information
Scopus Citations: 5
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