Deep glance on the antiparasitic anticancer activities of wheat germ oil in chronically infected immunosuppressed mice with cryptosporidiosis
Bibliographic Information
Authors: Abdelmaksoud H.F.; Aboushousha T.S.; El-Ashkar A.M.
Journal: Journal of Parasitic Diseases
Publisher: Springer
Publication Date: 2 June 2022
Volume / Issue: Volume 46 / Issue 3
Pages: 785–794
ISSN: 9717196
DOI: 10.1007/s12639-022-01497-z
Scopus: View on Scopus
Document Type: Article
Access: All Open Access; Green Open Access
Authors and Affiliations
Abdelmaksoud H.F., Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt; Aboushousha T.S., Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt; El-Ashkar A.M., Department of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt, Department of Basic Medical Science, College of Medicine, University of Bisha, Bisha, Saudi Arabia
Abstract
Cryptosporidium species are the major cause of water-borne epidemics of diarrhea in both developing and developed countries that vary from self-limited in immunocompetent patients to severe life-threatening in the immunocompromised hosts. There was a proven correlation between cryptosporidiosis and colorectal cancers, although, studies in this field are still limited. Wheat germ oil (WGO) is a natural product with a known antiparasitic effect and potential antiproliferative activities. This study aimed to evaluate the antiparasitic and anticancer activities of WGO in chronically infected immunosuppressed mice compared to Nitazoxanide (NTZ). This experimental case–control study was performed in the period from January till September 2021. Eighty immunosuppressed bred laboratory mice were divided into 4 groups, 20 mice each; GI non-infected; negative control (NC), GII infected non treated; positive control (PC), GII infected, and treated with NTZ, GIV infected, and treated with WGO. Parasitological, histopathological, and immunohistochemical examinations were performed with estimating the rate of maximal survival for the study groups. Parasitological examination revealed a marked reduction in the mean Cryptosporidium spp. oocyst counts in the stool of GIV compared to PC, and GIII (P-value < 0.001). Histopathological and immunohistochemical examinations showed the best results with GIV which revealed restoration of normal villous pattern, with no dysplasia or malignancy could be detected. GIV showed the best survival rate compared to PC and GIII. WGO is an extremely promising agent that has an excellent therapeutic effect against cryptosporidiosis with the ability to control the tumorigenesis process in the chronically infected immunosuppressed hosts. © 2022, Indian Society for Parasitology.
Keywords
Cryptosporidiosis; Histopathology; Immunohistochemistry; Nitazoxanide; Wheat germ oil; antineoplastic agent; antiparasitic agent; caspase 3; dexamethasone sodium phosphate; edible oil; eosin; hematoxylin; unclassified drug; animal experiment; animal model; animal tissue; antineoplastic activity; antiparasitic activity; Article; carcinogenesis; case control study; CD-1 mouse; chronic infection; controlled study; Cryptosporidium; female; immunosuppressive treatment; microscopy; mouse; nonhuman; oocyst; parasitology; survival analysis; survival rate; wheat germ
Citation Information
Scopus Citations: 7
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