Evaluation of the Potential Therapeutic Anti-inflammatory, Anti- proliferative and Apoptotic Effects of Viburnum tinus Leaves Different Extracts.

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Salman F.H.; Hassan E.A.; Mohamed M.A.; Sherif S.A.; Moharib M.N.; El Azeem E.M.A.

Journal: Egyptian Journal of Chemistry

Publisher: NIDOC (Nat.Inform.Document.Centre)

Publication Date: 26 October 2021

Volume / Issue: Volume 65 / Issue 4

Pages: 95–110

ISSN: 4492285

DOI: 10.21608/EJCHEM.2021.97025.4540

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Bronze Open Access

Authors and Affiliations

Salman F.H., Biochemistry and molecular biology department, TBRI, Giza, Egypt; Hassan E.A., Biochemistry department, Faculty of science, Ain Shams university, Cairo, Egypt; Mohamed M.A., Medicinal chemistry department, TBRI, Giza, Egypt; Sherif S.A., Biochemistry and molecular biology department, TBRI, Giza, Egypt; Moharib M.N., Biochemistry and molecular biology department, TBRI, Giza, Egypt; El Azeem E.M.A., Biochemistry department, Faculty of science, Ain Shams university, Cairo, Egypt

Abstract

Viburnum tinus (V.tinus) is an Adoxaceae family medicinal plant rich in vital phytochemicals such as polyphenols, triterpenoids, flavonoids, coumarins, and iridoids. This study shows that V. tinus leaf extracts have anti-inflammatory, anti-cancer, and apoptotic properties. The plant leaves were dried and soaked in 90% MeOH to prepare the crude extract, which was fractionated by n-Hexane, CH2Cl2, and MeOH. Phytochemicals were detected in these extracts, and their anti-inflammatory, anti-cancer, and apoptotic activities were evaluated in- vitro. The highest content of flavonoids, tannins, terpenoids, and coumarins in the n-Hexane extract stimulates its anti-inflammatory activity, as evidenced by protein denaturation inhibition (94.60±3.90 %), proteinase inhibition (88.5±0.50 %), membrane stabilization (73.2±1.58 %), and inflammatory cytokine (INF- γ) inhibition (100±0.00 %) without any toxicity on blood cells. It also has a cytotoxic impact on HepG2, MCF7, and CaCO2 cell lines, with IC50 (29.7 2.58, 75.3 4.70, 29.1 0.59 μg/ml) and SI (10.2, 10.4, 4) on HepG2, MCF7, and CaCO2, respectively. The apoptotic impact of n-Hexane in HepG2 resulted in quantitative expression of the BAX gene, which increased significantly and the BCL2 gene, which decreased significantly. © 2022 National Information and Documentation Center.

Keywords

Anti-cancer; Anti-inflammatory; Apoptosis; DNA fragmentation; INF-gamma; Pro and anti-apoptotic; Viburnum tinus

Citation Information

Scopus Citations: 3

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